Example sentences for: ecor

How can you use “ecor” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A partial human cDNA sequence of BRCA1 (from nucleotide 121 in exon 1 to nucleotide 1025 in exon 11, accession #U14680) was inserted in the antisense orientation into the EcoR1 site of the pLXSN retroviral vector (provided by AD Miller).

  • EcoR I adapter was added, the cDNA was digested by Xho I and the cDNA was inserted between the EcoR I and Xho I sites of the vectors in a directional fashion.

  • A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.

  • In the second step, the Nde I EcoR I fragment from pBS-NFATp(391-583) was ligated into the Nde I and EcoR I sites of pVL1392-GST.

  • pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.


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