Words similar to ecorv
Example sentences for: ecorv
How can you use “ecorv” in a sentence? Here are some example sentences to help you improve your vocabulary:
The 5' probe used detects a 11 kb wild type band and a 3.8 kb targeted band, due to the presence of an extra EcoRV site in the targeted allele.
Fig 3bshows that probe A identifies an 8.0 kb Hind III/EcoRV fragment in wild type ES cells.
Targeting of a pgkloxP-Neo cassette to this locus introduces a unique EcoRV site that generates a 4.0 kb HindIII/EcoRV fragment also detected by probe A. We obtained four correctly targeted ES cell clones out of 200 that were resistant to both G418 and gancyclovir (Fig 3b).
N3-F pCMV6 was made by PCR amplification of HsN3 (1-263) followed by subcloning the PCR products into the EcoR1/EcoRV sites of pFlag-CMV5C vector (Kodak).
Figs 3aand 3bshow that the genotype of ES cells at each stage of the procedure could be distinguished by genomic Southern blot analysis using a single DNA probe (probe A) to identify a Hind III/EcoRV restriction endonuclease fragment that was specific to each targeted allele.