Words similar to ecorv
Example sentences for: ecorv
How can you use “ecorv” in a sentence? Here are some example sentences to help you improve your vocabulary:
Genomic Southern blot hybridization was performed on DNA from ES cells digested with EcoRV.
Targeting of a pgkloxP-Neo cassette to this locus introduces a unique EcoRV site that generates a 4.0 kb HindIII/EcoRV fragment also detected by probe A. We obtained four correctly targeted ES cell clones out of 200 that were resistant to both G418 and gancyclovir (Fig 3b).
N3-F pCMV6 was made by PCR amplification of HsN3 (1-263) followed by subcloning the PCR products into the EcoR1/EcoRV sites of pFlag-CMV5C vector (Kodak).
A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.
Fig 3α shows that introduction of the LacZ transgene into the Hnf3 α locus was predicted to generate a 2.7 kb HindIII / EcoRV fragment that interacts with probe A. Fig 4shows that fifteen out of fifteen ES cell lines that were resistant to G418 had undergone homologous recombination and contained the LacZ transgene correctly targeted to the Hnf3 α locus.