Words similar to ecorv
Example sentences for: ecorv
How can you use “ecorv” in a sentence? Here are some example sentences to help you improve your vocabulary:
Southern blot analysis (fig 3) shows that deletion of the pgk promoter in Hnf3αloxPNeo cells generated a predicted 3.5 kb HindIII/EcoRV fragment that was detected by probe A. Moreover, Fig 3cshows that deletion of the pgk promoter resulted in the Hnf3αloxPNeo cells reverting to G418-sensitivity because the promoter is necessary for Neo expression.
Genomic Southern blot hybridization was performed on DNA from ES cells digested with EcoRV.
Fig 3bshows that probe A identifies an 8.0 kb Hind III/EcoRV fragment in wild type ES cells.
Targeting of a pgkloxP-Neo cassette to this locus introduces a unique EcoRV site that generates a 4.0 kb HindIII/EcoRV fragment also detected by probe A. We obtained four correctly targeted ES cell clones out of 200 that were resistant to both G418 and gancyclovir (Fig 3b).
The 5' probe used detects a 11 kb wild type band and a 3.8 kb targeted band, due to the presence of an extra EcoRV site in the targeted allele.