Words similar to ecorv

Example sentences for: ecorv

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sα i2 was excised from pSVsGi2 using EcoRI and SmaI and subcloned into the EcoRI and EcoRV sites of pcDNAI to produce sα i2 -pcDNAI.
Southern blot analysis (fig 3) shows that deletion of the pgk promoter in Hnf3αloxPNeo cells generated a predicted 3.5 kb HindIII/EcoRV fragment that was detected by probe A. Moreover, Fig 3cshows that deletion of the pgk promoter resulted in the Hnf3αloxPNeo cells reverting to G418-sensitivity because the promoter is necessary for Neo expression.
Fig 3α shows that introduction of the LacZ transgene into the Hnf3 α locus was predicted to generate a 2.7 kb HindIII / EcoRV fragment that interacts with probe A. Fig 4shows that fifteen out of fifteen ES cell lines that were resistant to G418 had undergone homologous recombination and contained the LacZ transgene correctly targeted to the Hnf3 α locus.
Targeting of a pgkloxP-Neo cassette to this locus introduces a unique EcoRV site that generates a 4.0 kb HindIII/EcoRV fragment also detected by probe A. We obtained four correctly targeted ES cell clones out of 200 that were resistant to both G418 and gancyclovir (Fig 3b).
N3-F pCMV6 was made by PCR amplification of HsN3 (1-263) followed by subcloning the PCR products into the EcoR1/EcoRV sites of pFlag-CMV5C vector (Kodak).