Example sentences for: ecorv

How can you use “ecorv” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Southern blot analysis (fig 3) shows that deletion of the pgk promoter in Hnf3αloxPNeo cells generated a predicted 3.5 kb HindIII/EcoRV fragment that was detected by probe A. Moreover, Fig 3cshows that deletion of the pgk promoter resulted in the Hnf3αloxPNeo cells reverting to G418-sensitivity because the promoter is necessary for Neo expression.

  • sα i2 was excised from pSVsGi2 using EcoRI and SmaI and subcloned into the EcoRI and EcoRV sites of pcDNAI to produce sα i2 -pcDNAI.

  • The C to T change at poition 1460 in exon 14 was genotyped according to [ 92 ] . Forward: 5'-TTAAATGGTCTCGGGAAGG-3' and Reverse 5'-GCCCAATGACACAGCCTTT-3' primers were used followed by digestion with EcoRV.

  • Targeting of a pgkloxP-Neo cassette to this locus introduces a unique EcoRV site that generates a 4.0 kb HindIII/EcoRV fragment also detected by probe A. We obtained four correctly targeted ES cell clones out of 200 that were resistant to both G418 and gancyclovir (Fig 3b).

  • Genomic Southern blot hybridization was performed on DNA from ES cells digested with EcoRV.


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