Words similar to ebpβ
Example sentences for: ebpβ
How can you use “ebpβ” in a sentence? Here are some example sentences to help you improve your vocabulary:
In this study, we show that p35-C/EBPβ and the TCF family member Elk-1 synergize in transactivation of SRF dependent transcription of both a Gal4 dependent reporter and an SRE-driven reporter construct, but only in response to mitogenic stimulation by Ras.
Therefore, we used a GST-pulldown assay to determine if the proteins could interact in vitro . p35-C/EBPβ was expressed as a chimeric GST protein and immobilized on glutathione-agarose beads.
However, the A-box deletion mutant, Elk-1(89-428), no longer binds to the GST-p35-C/EBPβ (lane 3, bottom panel).
We have shown that C/EBPβ interacts with the A-box of Elk-1, while SRF has previously been shown to interact with the B-box [ 16].
We further show that Elk-1 and p35-C/EBPβ interact in vitro in a glutathione-S-transferase (GST)-pulldown assay as well as in an in vivo coimmunoprecipitation assay.