Words similar to ebpα-gfp

Example sentences for: ebpα-gfp

How can you use “ebpα-gfp” in a sentence? Here are some example sentences to help you improve your vocabulary:

Approximately 1 × 10 7cells were transfected by electroporation, as previously described [ 42 ] , with 5 μg of either the C/EBPα-GFP, GFP-C/EBPα, or control expression vectors.
The cDNAs for the GFP-C/EBPα and C/EBPα-GFP fusion proteins were inserted into an expression vector and transfected into GHFT1-5 cells.
The C/EBPα-GFP vector was constructed by inserting the S65T GFP into the NcoI site present near the carboxy terminus of rat C/EBPα (also tagged at the amino terminus with the FLAG epitope) in our previously described C/EBPα expression vector [ 42 ] . This resulted in a deletion of the last four amino acids of C/EBPα.
The DBD and DBD + 154-257 derivatives of C/EBPα were constructed by replacing, respectively, the amino terminal 257 amino acids of C/EBPα-GFP (to the SgrAI site of C/EBPα) and the amino terminal 153 amino acids of C/EBPα-GFP (to the NotI site of C/EBPα) with oligonucleotides containing a strong Kozak sequence.
We determined if either C/EBPα-GFP or GFP-C/EBPα altered the distribution of GHFT1-5 cells in the G1, S or G2/M phases of the cell cycle.