Words similar to ebpα-gfp
Example sentences for: ebpα-gfp
How can you use “ebpα-gfp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Approximately 1 × 10 7cells were transfected by electroporation, as previously described [ 42 ] , with 5 μg of either the C/EBPα-GFP, GFP-C/EBPα, or control expression vectors.
C/EBPα-GFP Fusion protein with GFP attached to the carboxy terminus of C/EBPα
The C/EBPα-GFP vector was constructed by inserting the S65T GFP into the NcoI site present near the carboxy terminus of rat C/EBPα (also tagged at the amino terminus with the FLAG epitope) in our previously described C/EBPα expression vector [ 42 ] . This resulted in a deletion of the last four amino acids of C/EBPα.
The proportion of cells in G1 was not statistically different in cells expressing DBD + 1-154 from that in cells expressing full-length C/EBPα-GFP.
Flow cytometry (discussed later) showed that comparable levels of green fluorescence were emitted from cells expressing C/EBPα-GFP, GFP-C/EBPα and ΔLZ.