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Example sentences for: dt

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As proof that the (K395A/3F)Syk molecule was inactive as predicted, (K395A/3F)Syk and wildtype Syk expression constructs were transfected into syk -negative DT40 cells, and activity measured by the ICKA (Figure 5).
Approximately 10 μg of purified RNA was used to prepare double-stranded cDNA (Supercript GIBCO/BRL, Rockville, MD) using a T7 (dT)24 primer containing a T7 RNA polymerase promoter binding site.
A major biological response of B cells to BCR cross-linking is apoptosis, a function that is strongly induced in DT40 cells.
Because stable hybridization duplexes have been reported with oligonucleotides as short as 7 bases [ 5 ] , and because such stable duplexes may be formed even if there are base-pair mismatches [ 6 ] , it is conceivable that the oligo(dT) probe could have hybridized to the A-rich regions in the 3' or 5' ends of the DNA in each the outlier spots.
The Btk:ER constructs were transfected into Btk-deficient DT40 chicken B cells and stable clones were selected that expressed equivalent levels of Btk:ER.