Words similar to dominant-negative
Example sentences for: dominant-negative
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Previous studies have shown that Rab24 exhibits several unusual characteristics that distinguish it from other members of the Rab GTPase family [ 25 ] . The studies described in this report demonstrate that putative dominant-negative mutant forms of Rab24, bearing single amino acid substitutions in the conserved N(T)KxD nucleotide binding motif ( e.g. , D123I and T120A), are targeted to numerous inclusion bodies that disrupt the nuclear envelope and enter the nuclear compartment.
One gene in this region, slimb , is an interesting candidate because this gene encodes an F-box protein that is part of an SCF complex [ 26 ] [ 29 ] . Several independent alleles of slimb were tested and all enhanced the dominant-negative mCul1 overexpression phenotype (fig.
A partial cDNA clone homologous to AtCOP1 has been previously identified in mammals, containing all three conserved protein-protein interaction domains [ 20 ] . Remarkably, when expressed in plant cells, the mammalian COP1-reporter fusion protein exhibited a light-regulated nuclear localization pattern similar to AtCOP1 [ 20 ] . Although mammalian COP1 failed to rescue the Arabidopsis cop1 mutants, over-expression of the amino-terminal half of mammalian COP1 in Arabidopsis caused a dominant-negative phenotype, resembling the effect of over-expressing the corresponding fragment of AtCOP1 [ 20 21 ] .
Although the initial cloning of ASW pre-dated knowledge that Hint is an enzyme, both groups suggested that Asw might function by a dominant-negative mechanism through heterodimerization with Hint, thereby inducing female development [ 33, 34].
We attempted to distinguish between these possibilities by using an alternative strategy to make a dominant-negative form of Rab24.
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