Words similar to dnase
Example sentences for: dnase
How can you use “dnase” in a sentence? Here are some example sentences to help you improve your vocabulary:
DNase I footprints were performed with a 187 bp DNA fragment containing the -111 to +42 region of the human IL-2 promoter and some surrounding vector DNA.
Nucleic acid contamination was considered to be minimal due to the repeated DNase and RNase treatments.
Reverse transcription of 2 μg of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II RNase H-Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 500 ng of random hexamers.
Tissue from each organ was extracted from 5 individual mice and extracted separately using Qiagen RNeasy mini-kits (Basel, Switzerland) according to manufacturer's instructions with one exception: During extractions, all RNeasy columns were impregnated with DNase I (Roche, Basel, Switzerland) to remove possible genomic DNA contamination.
5 target sites like other Fnr-regulated class II promoters [ 16 ] . By analogy to the CAP transcription regulator [ 17 18 ] , the proposed mechanism for Fnr activation of gene expression involves its ability to promote open complex formation by RNA polymerase [ 13 16 19 ] . As suggested by the appearance of DNase I hypersensitive cleavage sites, a change in the DNA conformation occurs upon Fnr binding at the dmsA promoter [ 7 8 ] . The presence of hypersensitive DNase I cleavage sites at other FNR-regulated promoters as well as DNA bending experiments support this conclusion [ 8 20 ] . Furthermore, recent studies propose the protein-protein interaction of σ 70and Fnr at the narG and dmsA promoters [ 7 21 22 23 ] . Finally, for each of the site-directed mutations of the Fnr binding site in this study, the level of dmsA-lacZ expression was not significantly altered in an fnr deletion strain either aerobically or anaerobically, indicating that the Fnr-independent expression from the dmsA promoter was not affected by the sequence alterations (Figure 1).
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