Words similar to dnase
Example sentences for: dnase
How can you use “dnase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cell culture reagents and enzymes including MEM, HAM's F12, D-valine, dialyzed fetal calf serum, Pronase and DNase I are from Life Technologies (Grand Island, NY) except as noted.
We then demonstrated that the shared molegos served a similar role in substrate binding by comparing the DNA binding profile of huAPE1 with that for the less specific enzyme DNase 1. The molegos present in both enzymes interact with target DNA in a similar fashion, while residues in molegos distinctive for APE1 control specificity by binding primarily to the bases around the apurinic site.
The labeled cRNA was purified using RNeasy spin columns (Qiagen, Chatsworth, CA), followed by DNase I treatment and a second round of RNeasy spin-column purification.
Reverse transcription of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II Rnase H -Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 2 μg of total RNA and 1 μg of random hexamers.
Choroid plexus cells were isolated as described [ 46 ] . Briefly, choroid plexus from adult male Sprague-Dawley rats (175-200 g) were dissected into cold Locke's buffer, rinsed and enzymatically dissociated for 10 minutes at 37° C in Hank's Balanced Salt Solution (HBSS) containing 330 ug/ml Pronase I and 260 ug/ml DNase I. The cells were pelleted and choroid plexus cells were released after an additional digest with 130 ug/ ml DNase I in HBSS.
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