Words similar to dnase
Example sentences for: dnase
How can you use “dnase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Reverse transcription of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II Rnase H -Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 2 μg of total RNA and 1 μg of random hexamers.
, the segmental RMSD's) between APE1 and representatives of the distantly related DNase 1 and IPP families are comparable to those found between members of the APE family (Figure 4and Table 1& 2).
0 g of wet weight bacteria/30 ml of buffer) that contained 10 mM Tris-hydrochloride, 2 mM MgCl 2 , 100 μg/ml DNase I, and 25 μg/ml RNase I. The slurry was passed twice through a French Press set at 20,000 lbs/in 2. DNase and RNase were again added, and the mixture was incubated at 37°C for 2 hours.
Earlier studies have shown that DAP, in DNA fragments, can widen the minor groove, as detected by reactivity towards uranyl nitrate or susceptibility to Dnase cleavage [ 29 ] , and can relieve compressions in the minor groove associated with A-tracts [ 30 ] . In contrast, NMR and molecular modeling studies of an oligonucleotide duplex containing MeC have demonstrated that, while this duplex still has a B-DNA conformation, there are differences in the structural parameters and thermal stability relative to the unmodified duplex [ 31 ] . An earlier study also found, in two closely related octanucleotide duplexes, that although the methylated duplexes retained their B-DNA conformation, different structural and thermal stability effects were seen [ 32 ] . Although these studies have been carried out with DNA-DNA and not DNA-RNA duplexes, it is possible that MeC substitution may have different structural consequences than DAP substitution.
DNase (GIBCO BRL, 295 units) was added and the reaction was incubated at 37°C for an additional 30 minutes.
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