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Example sentences for: dnasei

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2) can be identified, which are strictly invariant among all the yCCR4-related proteins, but have no equivalent in the presently aligned AP-endonucleases and DNAseI, or in other Mg 2+-dependent endonuclease fold-containing proteins previously described (not shown) [ 12 13 ] . These CCR4-specific amino acids are most probably relevant to functions common to and specific for all yCCR4-related proteins (see Discussion).
RNA was isolated and the genomic DNA was depleted by using DNaseI (Amplification Grade, GIBCO BRL) following the manufacturer's protocols.
In addition, the putative active residues shared by both AP endonucleases and DNAseI [ 29 30 31 32 ] , are strictly conserved in all the yCCR4-related proteins: these are residues involved in catalysis (indicated by triangles in Fig.
Two micrograms of the extracted RNA was treated with 2 U DNaseI to eliminate DNA, and reverse transcribed with 200 U SuperScriptII reverse transcriptase at 42°C for 70 min using oligo dT primers in accordance with the manufacturer's instructions.
Unlike DNaseI, 1,10-phenanthroline-cuprous complex is a small chemical probe which can demark the boundaries of the protected region clearly.