Example sentences for: dnasei

How can you use “dnasei” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Two micrograms of the extracted RNA was treated with 2 U DNaseI to eliminate DNA, and reverse transcribed with 200 U SuperScriptII reverse transcriptase at 42°C for 70 min using oligo dT primers in accordance with the manufacturer's instructions.

  • 2) can be identified, which are strictly invariant among all the yCCR4-related proteins, but have no equivalent in the presently aligned AP-endonucleases and DNAseI, or in other Mg 2+-dependent endonuclease fold-containing proteins previously described (not shown) [ 12 13 ] . These CCR4-specific amino acids are most probably relevant to functions common to and specific for all yCCR4-related proteins (see Discussion).

  • 2) on ice for 2 min, and then treated with 50 units of DNaseI at 37°C for 10 min.

  • In addition, the putative active residues shared by both AP endonucleases and DNAseI [ 29 30 31 32 ] , are strictly conserved in all the yCCR4-related proteins: these are residues involved in catalysis (indicated by triangles in Fig.

  • RNA was isolated and the genomic DNA was depleted by using DNaseI (Amplification Grade, GIBCO BRL) following the manufacturer's protocols.


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