Words similar to dnd-

Example sentences for: dnd-

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In order to stain acidic lysosomes, LysoTracker Red DND-99 was used.
OxyBURST Green H 2 HFF BSA, dextrans (10 K, lysine fixable) conjugated with either Cascade Blue or fluorescein fluorophores, LysoTracker Red DND-99 and 5-(and -6)-carboxyfluorescein were from Molecular Probes, Inc. (Eugene, OR).
The effects of H 2 O 2 and O 2 •-on the fluorescence of LysoTracker Red DND-99 were studied by preparing 2 μM solutions of the dye in MES buffer solutions at pH 4.5 or 7.5, containing various concentrations of H 2 O 2 and KO 2 . After incubation at room temperature for 10 min, the emission fluorescence at λ 592 was recorded as previously described.
LysoTracker Red DND-99 is a chloroquine analogue that had been reported to selectively accumulate in acidic lysosomes [ 9 ] . In the present study, when lysosomal pH was elevated to >6.
Cells were then treated with 200 nM PMA for 30 min, followed by 25 nM LysoTracker Red DND-99 for 5 min to label lysosomes.