Words similar to dnd-
Example sentences for: dnd-
How can you use “dnd-” in a sentence? Here are some example sentences to help you improve your vocabulary:
In order to stain acidic lysosomes, LysoTracker Red DND-99 was used.
The LysoTracker Red DND-99 is a fluorescent analog of chloroquine, which selectively accumulates in lysosomes [ 9 ] . Cells were incubated with a 25 nM concentration of the dye at 37°C for 5 min.
The effects of H 2 O 2 and O 2 •-on the fluorescence of LysoTracker Red DND-99 were studied by preparing 2 μM solutions of the dye in MES buffer solutions at pH 4.5 or 7.5, containing various concentrations of H 2 O 2 and KO 2 . After incubation at room temperature for 10 min, the emission fluorescence at λ 592 was recorded as previously described.
The effect of pH on the fluorescence of LysoTracker Red DND-99 (final concentration is 2 μM) was similarly performed, except that the emission fluorescence at λ 592 was recorded upon excitation at λ 574 .
Cells were then treated with 200 nM PMA for 30 min, followed by 25 nM LysoTracker Red DND-99 for 5 min to label lysosomes.