Words similar to dmsabc
Example sentences for: dmsabc
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Mutagenesis of the Fnr recognition sequence at the dmsA promoter region confirmed the contribution of Fnr in the 100-fold activation of dmsABC expression and explored the DNA sequence and spacing requirements for the Fnr recognition site at the P1 promoter.
The enhanced ability of Fnr to activate dmsABC gene expression is possibly, by analogy to Crp, due to increased affinity of Fnr at the consensus versus the native non-consensus Fnr recognition sequence at dmsA . Likewise, the poor match of the Fnr site at the promoter for the fumarate reductase gene, frdA to the consensus Fnr sequence may account for the relatively weak anaerobic induction for this respiratory operon [ 14 ] .
NarL-phosphate then negatively regulates dmsABC operon expression [ 4 ] . Additional regulators of dmsABC expression have also been described that involve molybdenum dependent gene expression via the ModE transcription factor, and integration host factor (IHF) that interacts with DNA at a downstream site called P2 [ 4 6 11 12 ] . Neither the presence of TMAO nor DMSO, substrates for the DMSO reductase, further modulate dmsABC gene expression [ 4 6 ] .
This study investigated the effects of sequence changes in the Fnr-recognition site on the anaerobic activation of dmsA-lacZ expression as well as examined the NarL recognition sites within the dmsABC regulatory region.
Furthermore, the spacing between the Fnr and RNA polymerase recognition sequences is critical at dmsA . In vitro interactions of the nitrate-responsive regulatory protein NarL with the promoter region of dmsABC were examined using DNase I and hydroxyl radical footprinting techniques.
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