Words similar to dmsabc
Example sentences for: dmsabc
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These studies define the molecular interactions of Fnr and NarL at the dmsABC P1 promoter that together provide for the oxygen and nitrate regulated expression of this respiratory pathway operon.
The enhanced ability of Fnr to activate dmsABC gene expression is possibly, by analogy to Crp, due to increased affinity of Fnr at the consensus versus the native non-consensus Fnr recognition sequence at dmsA . Likewise, the poor match of the Fnr site at the promoter for the fumarate reductase gene, frdA to the consensus Fnr sequence may account for the relatively weak anaerobic induction for this respiratory operon [ 14 ] .
This study investigated the effects of sequence changes in the Fnr-recognition site on the anaerobic activation of dmsA-lacZ expression as well as examined the NarL recognition sites within the dmsABC regulatory region.
5 bp from the dmsABC P1 promoter as also established by DNase I footprinting analyses [ 7 8 ] . The presence of nitrate, a preferred alternative electron acceptor, elicits a 10-fold repression of dmsABC expression via the NarXLQP two-component regulatory system [ 4 9 10 ] . Nitrate is detected by either of the two sensor-transmitter proteins, NarX or NarQ, which then activate the DNA binding response-regulatory protein NarL, by phosphorylating an aspartate residue located at position 59.
Mutagenesis of the Fnr recognition sequence at the dmsA promoter region confirmed the contribution of Fnr in the 100-fold activation of dmsABC expression and explored the DNA sequence and spacing requirements for the Fnr recognition site at the P1 promoter.
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