Words similar to dmsa
Example sentences for: dmsa
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These findings demonstrate that the smaller dmsA region containing only one of the three consensus heptamer sites (Figure 4) is sufficient for NarL binding.
This signifies that NarL-phosphate binds to only one side of the DNA molecule dmsA promoter region [ 27 28 29 30 ] . The hydroxyl radical data are also consistent with the binding of multiple molecules of NarL-phosphate to the dmsA promoter region as suggested by the DNase I data.
The region of dmsA promoter DNA protected by the NarL regulatory protein was identified by DNase I and hydroxyl radical footprinting experiments.
Three putative NarL binding sites with the consensus heptameric sequence (TACYYMT) have been proposed for dmsA [ 26 ] . These sites, previously assigned at positions +8, -14 and -34, are now centered at positions + 15, -7, and -27 (Figure 4) due to the reassignment of the dmsA P1 start site [ 5 6 ] . Since the size of the DNase I and hydroxyl radical footprints in this study show DNA protections between and beyond these three consensus sites, other NarL binding sites may be present in this region.
The protections are consistent with the proposal that NarL-phosphate recognizes and binds at multiple heptamer recognition sites within the dmsA P1 promoter region.
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