Words similar to dmsa
Example sentences for: dmsa
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5 target sites like other Fnr-regulated class II promoters [ 16 ] . By analogy to the CAP transcription regulator [ 17 18 ] , the proposed mechanism for Fnr activation of gene expression involves its ability to promote open complex formation by RNA polymerase [ 13 16 19 ] . As suggested by the appearance of DNase I hypersensitive cleavage sites, a change in the DNA conformation occurs upon Fnr binding at the dmsA promoter [ 7 8 ] . The presence of hypersensitive DNase I cleavage sites at other FNR-regulated promoters as well as DNA bending experiments support this conclusion [ 8 20 ] . Furthermore, recent studies propose the protein-protein interaction of σ 70and Fnr at the narG and dmsA promoters [ 7 21 22 23 ] . Finally, for each of the site-directed mutations of the Fnr binding site in this study, the level of dmsA-lacZ expression was not significantly altered in an fnr deletion strain either aerobically or anaerobically, indicating that the Fnr-independent expression from the dmsA promoter was not affected by the sequence alterations (Figure 1).
As the three consensus NarL boxes flank the dmsA promoter and are spaced 20 bp apart (Figure 4), the spacing and orientation of the NarL protected regions make it tempting to speculate that NarL-phosphate binds at each site.
Furthermore, non-phosphorylated NarL gave no DNase I protections in the dmsA promoter region under the same conditions for either strand (up to 10 μM), suggesting that NarL-phosphate, but not NarL, can act as a transcriptional repressor for dmsA expression.
The NarL interactions with the dmsA promoter region occurred at ten bp intervals and were offset by 3 bp in the 3' direction, suggesting the assembly of multiple NarL-phosphate molecules onto one face of the DNA that protect the minor groove.
In contrast, the full-length dmsA fragment (Fragment A, -127 to +62) showed a larger protected region from -51 to +32 (lanes 2-5).
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