Words similar to dmsa

Example sentences for: dmsa

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Mutagenesis of the Fnr recognition sequence at the dmsA promoter region confirmed the contribution of Fnr in the 100-fold activation of dmsABC expression and explored the DNA sequence and spacing requirements for the Fnr recognition site at the P1 promoter.
Therefore, a simple model that accounts for the footprint data is the assembly of multiple NarL-phosphate molecules onto one face of the DNA that somehow protect the minor groove from hydroxyl radical attack [ 28 29 30 ] . In an alternative model, NarL binds only at the three proposed NarL heptamer consensus sites spaced at 20 bp intervals within the dmsA regulatory region (Figure 4).
Each intended mutation was confirmed by DNA sequence analysis [ 34 ] . A Hinc II/ Bam HI fragment containing the regulatory region was excised from the M13 template and inserted into the plasmid pRS1274 to give the JA250 (wild type) and related mutant dmsA - lac Z promoter fusions (Figure 1).
The DNase I footprint of the non-coding strand of dmsA with phosphorylated NarL revealed an 83 bp protected region that extends from -51 to +32 relative to the start of transcription (Figure 3).
Hydroxyl radical footprinting at the dmsA promoter was performed as described [ 27 ] . NarL binding to the indicated DNA fragment was performed as described above, omitting glycerol from the binding buffer.