Words similar to dmsa
Example sentences for: dmsa
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The hydroxyl radical protected regions for the dmsA strands of DNA were offset by 3 bp in the 3' direction (Figure 4).
As the three consensus NarL boxes flank the dmsA promoter and are spaced 20 bp apart (Figure 4), the spacing and orientation of the NarL protected regions make it tempting to speculate that NarL-phosphate binds at each site.
To our knowledge, this "consensus" Fnr-dependent dmsA promoter exhibits the highest anaerobic induction of any Fnr-regulated E. coli promoter examined.
Three putative NarL binding sites with the consensus heptameric sequence (TACYYMT) have been proposed for dmsA [ 26 ] . These sites, previously assigned at positions +8, -14 and -34, are now centered at positions + 15, -7, and -27 (Figure 4) due to the reassignment of the dmsA P1 start site [ 5 6 ] . Since the size of the DNase I and hydroxyl radical footprints in this study show DNA protections between and beyond these three consensus sites, other NarL binding sites may be present in this region.
However, a somewhat weaker binding of NarL-phosphate to the DNA fragment containing the truncated dmsA region relative to the full-length region may suggest that NarL binds at the promoter in a weakly cooperative fashion.