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Example sentences for: diploids

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At present, two hypothetical mechanisms by which a Rec12-dependent signal affects MII are proposed: First, Rec12-dependent (recombination-dependent) signals might trigger a block after MI, thereby allowing cells to skip a frequently irregular MII division [ 39 ] . However, the majority of the rec12 mutant meioses show evidence of two meiotic divisions (Figure 3) and many of the meiotic diploids exhibit mixed MI and MII missegregation patterns for centromere linked markers on the three different chromosomes (Table 2).
We used Southern blotting to determine the genotype of one randomly selected Arg +Ura +derivative from each of the 54 separate cultures of the heterozygote diploids.
The diploids were sporulated, and if ~50% of the MEN-deleted spores were resistant to 5-FOA, then net1-1 could bypass the MEN null mutation.
Expression of the capping enzymes from these plasmids is driven by the nmt1* promoter [ 19 ] . The plasmids were transformed into heterozygous pct1 +/ pct1::kanMX or pce1 +/ pct1::kanMX diploids using the lithium acetate method [ 18 ] . The Leu +diploid transformants were then sporulated on ME plates at room temperature.
Thus, those meioses did not exhibit simple MI nondisjunction, because 100% of diploids arising from MI nondisjunction would be heterozygous at the centromeres (Figure 5B).