Words similar to derived
Example sentences for: derived
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Nagy et al demonstrated that embryos and mice could be derived solely from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . Although we were able to repeat these data using R1 ES cells we were unable to generate midgestation embryos from E14TG2a-derived strains of hprt negative ES cell lines using the same approach [ 9 10 11 ] . We believed it was unlikely that Hprt itself was required to generate ES cell-derived embryos because hprt knockout mice are viable and fertile [ 12 ] . E14TG2a-derived ES cell lines contain a large spontaneous deletion encompassing 55 kb of the hprt locus [ 13 ] . Therefore, it seemed possible that sequences within this region were necessary to maintain ES cell pluripotency.
The mammalian BDNF gene is known to have at least four promoters whose cell-specific and developmental activity is differentially regulated [ 20 21 22 23 24 25 26 ] . The transcripts derived from these promoters have a common organization.
The dried DNA was dissolved in 2 μl of loading buffer (7 M Urea, 5 mM EDTA, 0.5% Blue Dextrane, pH 8.0) containing a ROX-labelled size marker derived from specifically designed λ-phage PCR fragments (45 bp, 80 bp, 120 bp, 160 bp, 200 bp, 240 bp, 280 bp, 320 bp, 360 bp, 400 bp, 450 bp, 500 bp, 550 bp, 600 bp, 650 bp, 700 bp, 750 bp, 800 bp, 850 bp, 900 bp).
Indeed, a phylogenetic analysis of the envelope protein of these elements reported by Malik et al [ 23] suggests that they are derived from a monophyletic event that resulted from their incorporation of a baculovirus envelope protein gene in an ancestral member of these retrovirus-like elements.
The plasmid containing FS was constructed by sub-cloning of the XbaI-PstI fragment derived for rat FS cDNA clone [ 9 ] , pROF304 into pBluescript SK+.
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