Words similar to dephosphorylated
Example sentences for: dephosphorylated
How can you use “dephosphorylated” in a sentence? Here are some example sentences to help you improve your vocabulary:
We first determined the effect of SKP2 overexpression on MIB-induced RB1 downregulation and accumulation in the dephosphorylated state (see Fig.
As shown in Figure 5B, the majority of phosphorylated HA-NFATp was found in the supernatant and not in the pellet, while approximately 50% of the dephosphorylated NFATp was found associated with nuclei.
A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.
Similarly, when NFATp is purified from unstimulated T cells and subsequently dephosphorylated by calcineurin in vitro, its migration on SDS gels increases (i.e.
In vitro studies have demonstrated that dephosphorylated cPLA 2 is catalytically active and that Ser 505phosphorylation increases activity by only ~30 percent [ 24 ] . In contrast, cells expressing the cPLA 2 S505A mutation fail to release AA in response to a low dose of calcium ionophore, but release similar amounts of AA as cells expressing wild-type cPLA 2 in response to high dose ionophore [ 22 ] . From these studies, it has been suggested that cPLA 2 Ser 505phosphorylation may have a role in regulating translocation [ 22 ] . A previous study demonstrated translocation of cPLA 2 S505A in response to Ca 2+ionophore, but did not address the kinetics of translocation, translocation in response to a physiological agonist, or differences in targeting [ 25 ] .
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