Words similar to dephosphorylated
Example sentences for: dephosphorylated
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As shown in Figure 5B, the majority of phosphorylated HA-NFATp was found in the supernatant and not in the pellet, while approximately 50% of the dephosphorylated NFATp was found associated with nuclei.
Threonine558-phosphomoesin was not dephosphorylated in these fixatives, as determined by western blotting with specific phosphomoesin antibodies (Figure 6B).
As shown here, however, moesin apparently was not dephosphorylated in PFA-based fixatives in NIH3T3 cells.
In vitro studies have demonstrated that dephosphorylated cPLA 2 is catalytically active and that Ser 505phosphorylation increases activity by only ~30 percent [ 24 ] . In contrast, cells expressing the cPLA 2 S505A mutation fail to release AA in response to a low dose of calcium ionophore, but release similar amounts of AA as cells expressing wild-type cPLA 2 in response to high dose ionophore [ 22 ] . From these studies, it has been suggested that cPLA 2 Ser 505phosphorylation may have a role in regulating translocation [ 22 ] . A previous study demonstrated translocation of cPLA 2 S505A in response to Ca 2+ionophore, but did not address the kinetics of translocation, translocation in response to a physiological agonist, or differences in targeting [ 25 ] .
When T cells are stimulated, NFATp is dephosphorylated by calcineurin and it migrates farther on SDS gels than its phosphorylated counterpart.
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