Words similar to dephosphorylated
Example sentences for: dephosphorylated
How can you use “dephosphorylated” in a sentence? Here are some example sentences to help you improve your vocabulary:
To examine whether the p44/42 MAPK pathway mediates insulin signaling in the H441 cells, PD98059, an inhibitor of the p44/42 MAPK pathway, was used [ 9 ] . PD98059 binds to the dephosphorylated form of MEK and prevents its activation in vivo with an IC 50 of 10 μM [ 9 ] . PD98059, added alone, significantly decreased SP-A mRNA levels in a dose-dependent manner (Fig.
The resulting vector was digested with EcoRI, dephosphorylated with calf intestinal phosphatase (CIP, NEB), and a PCR product generated from pVT27 (Abedi et.
Focal adhesion kinase (FAK) is a known substrate for PTEN and has been shown to be dephosphorylated by this phosphatase in vitro and in vivo [ 20 21 34 ] . We therefore determined whether the increased PTEN phosphatase activity would correlate with decreased phosphorylation of FAK in our cells.
We first determined the effect of SKP2 overexpression on MIB-induced RB1 downregulation and accumulation in the dephosphorylated state (see Fig.
In another report, micro-injection of PP1 decreased extractability of pRB from nuclei, presumably by increasing dephosphorylation and increasing association with nuclear proteins thereby playing a role in G1/S transition inhibition [ 13 ] . Micro-injection of antibodies to the PP1 catalytic subunit has been shown to block cells in metaphase [ 23 ] . Since this is the time during mitosis when pRB is normally dephosphorylated [ 11 24 ] , it is tempting to speculate that prevention of pRB dephosphorylation by inhibiting PP1 activity impedes an orderly progression through mitosis, perhaps contributing towards a transformed phenotype.
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