Words similar to dephosphorylated
Example sentences for: dephosphorylated
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A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.
In another report, micro-injection of PP1 decreased extractability of pRB from nuclei, presumably by increasing dephosphorylation and increasing association with nuclear proteins thereby playing a role in G1/S transition inhibition [ 13 ] . Micro-injection of antibodies to the PP1 catalytic subunit has been shown to block cells in metaphase [ 23 ] . Since this is the time during mitosis when pRB is normally dephosphorylated [ 11 24 ] , it is tempting to speculate that prevention of pRB dephosphorylation by inhibiting PP1 activity impedes an orderly progression through mitosis, perhaps contributing towards a transformed phenotype.
The resulting vector was digested with EcoRI, dephosphorylated with calf intestinal phosphatase (CIP, NEB), and a PCR product generated from pVT27 (Abedi et.
We first determined the effect of SKP2 overexpression on MIB-induced RB1 downregulation and accumulation in the dephosphorylated state (see Fig.
As shown in Figure 5B, the majority of phosphorylated HA-NFATp was found in the supernatant and not in the pellet, while approximately 50% of the dephosphorylated NFATp was found associated with nuclei.
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