Words similar to dephosphorylate

Example sentences for: dephosphorylate

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Our second approach involved testing immunopurified 6His-HA-PP1α for its ability to dephosphorylate the growth suppressor protein pRB.
Having previously reported on the ability of immunocomplexed endogenous PP1 to dephosphorylate pRB [ 20 ] , we followed the same experimental approach.
First, immunocomplexed 6His-HA-PP1α was tested for its ability to dephosphorylate phosphorylase a . Phosphorylated on a single serine residue, this substrate is often used to monitor the biochemical activity of PP1 in vitro [ 28 ] . As shown in Figure 5, greater phosphatase activity is detected in immunoprecipitates from induced LLWO2F cell lysates compared to uninduced.
To inhibit any endogenous PP2A activity, which can also dephosphorylate phosphorylase a and thus interfere with PP1 activity measurements, these assays were carried out in the presence of 5 nM okadaic acid, which does not inhibit PP1 activity [ 30 ] . Although reproducible, we observed only modest increases in PP1-specific activity found in lysates from induced cells compared to the uninduced controls (Figure 7).