Example sentences for: depc-treated

How can you use “depc-treated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After in vitro transcription, 20 U of RNase-free DNase I was added to digest the DNA templates, and the labeled riboprobes were precipitated with ethanol and then resuspended in 100 μl of DEPC-treated water.

  • Total RNA was extracted by the TRIZOL reagent protocol (GIBCO/BRL, Rockville, MD), and then dissolved in DEPC-treated water before storage at -80°C.

  • Tissue sections were fixed for 30 min in 4% paraformaldehyde in DEPC-treated PBS, washed twice for 15 min in PBS containing 0.1% active DEPC, and equilibrated for 15 min in DEPC-treated 5x SSC.

  • HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.

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