Words similar to depc
Example sentences for: depc
How can you use “depc” in a sentence? Here are some example sentences to help you improve your vocabulary:
For cDNA Expression Array analysis, E15 SMG primordia were cultured in the presence or absence of SN50 peptide for 2 days (E15 + 2), collected in cold PBS containing 0.02% DEPC, snap frozen, and stored at -70°C.
Samples were diluted in depC H 2 O and 50 ng were used in quantitative one-tube reverse-transcription PCR (Applied Biosystems, Foster City, CA, USA).
For the mRNA pans, all solutions and surfaces are pretreated with DEPC or RNaseZap (Ambion, Austin TX), respectively, to eliminate RNase contamination that may compromise the integrity of the RNA.
RNA samples (control and experimental) were each mixed with random octamers in a total volume of 25 μl DEPC (diethylpyrocarbonate) treated water, heated to 65°C for 5 minutes and then transferred to 42°C.
mRNA was added to 4 μg of oligo (dT21) primer (Life Technologies, Rockville, MD), DEPC water was used to bring the volume to 27 μl and the mixture was incubated at 65°C for 10 min and placed on ice.
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