Example sentences for: depc

How can you use “depc” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • mRNA was added to 4 μg of oligo (dT21) primer (Life Technologies, Rockville, MD), DEPC water was used to bring the volume to 27 μl and the mixture was incubated at 65°C for 10 min and placed on ice.

  • Total RNA was extracted from 100 mg of frozen lung using Tri-reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and the final product was resuspended in 20 μl diethylpyro carbonate depC H 2 O. RNA was quantified and samples with A 260 /A 280 >1.

  • Tissue sections were fixed for 30 min in 4% paraformaldehyde in DEPC-treated PBS, washed twice for 15 min in PBS containing 0.1% active DEPC, and equilibrated for 15 min in DEPC-treated 5x SSC.

  • No.A-5660]), 10 μl DEPC treated water, and 2 μl Superscript II was added to each tube.

  • For the mRNA pans, all solutions and surfaces are pretreated with DEPC or RNaseZap (Ambion, Austin TX), respectively, to eliminate RNase contamination that may compromise the integrity of the RNA.

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