Words similar to deparaffinized
Example sentences for: deparaffinized
How can you use “deparaffinized” in a sentence? Here are some example sentences to help you improve your vocabulary:
Then, each antibody was tested with our published protocol, which involves an overnight incubation of deparaffinized sections at 70°C in the retrieval solution and an incubation for 3-4 hours or overnight of the primary antibody at about 25°C [ 26 ] . Two adjacent sections stained by using our protocol and the manufacturer's recommended protocols were compared for specificity and sensitivity.
025% chromium potassium sulfate, 0.25% gelatin), air-dried, deparaffinized, rehydrated, and stained with hematoxylin and eosin (H & E) or processed for flourescence microscopy (Olympus BH-2, Tokyo, Japan).
Briefly, sections were deparaffinized, and those to be stained with Ki-67 were subjected to microwave antigen retrieval in citrate buffer for 10 minutes, twice.
Sections were deparaffinized in 3 changes of Hemo-De for 15 min each and rehydrated in a descending ethanol series (5 min each: 100%, 95%, 70%, 50%, 30% ethanol).
Briefly, 5 μm sequential sections were deparaffinized and fixed with 4% paraformaldehyde for 20 minutes at room temperature followed by 30 minute wash in PBS.