Words similar to deparaffinized
Example sentences for: deparaffinized
How can you use “deparaffinized” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, sections were deparaffinized, and those to be stained with Ki-67 were subjected to microwave antigen retrieval in citrate buffer for 10 minutes, twice.
Formalin fixed, paraffin embedded mouse brain sections were deparaffinized and antigen retrieval was performed for 1 minute in a pressure cooker with antigen unmasking solution (Vector Laboratories, Burlingame, CA).
Briefly, 5 μm sequential sections were deparaffinized and fixed with 4% paraformaldehyde for 20 minutes at room temperature followed by 30 minute wash in PBS.
Paraffin blocks were cut in 5-6 μm sections, deparaffinized and hydrated to water prior to staining.
Then, each antibody was tested with our published protocol, which involves an overnight incubation of deparaffinized sections at 70°C in the retrieval solution and an incubation for 3-4 hours or overnight of the primary antibody at about 25°C [ 26 ] . Two adjacent sections stained by using our protocol and the manufacturer's recommended protocols were compared for specificity and sensitivity.