Example sentences for: deparaffinized

How can you use “deparaffinized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sections were deparaffinized in 3 changes of Hemo-De for 15 min each and rehydrated in a descending ethanol series (5 min each: 100%, 95%, 70%, 50%, 30% ethanol).

  • Briefly, 5 μm sequential sections were deparaffinized and fixed with 4% paraformaldehyde for 20 minutes at room temperature followed by 30 minute wash in PBS.

  • 025% chromium potassium sulfate, 0.25% gelatin), air-dried, deparaffinized, rehydrated, and stained with hematoxylin and eosin (H & E) or processed for flourescence microscopy (Olympus BH-2, Tokyo, Japan).

  • Formalin fixed, paraffin embedded mouse brain sections were deparaffinized and antigen retrieval was performed for 1 minute in a pressure cooker with antigen unmasking solution (Vector Laboratories, Burlingame, CA).

  • Then, each antibody was tested with our published protocol, which involves an overnight incubation of deparaffinized sections at 70°C in the retrieval solution and an incubation for 3-4 hours or overnight of the primary antibody at about 25°C [ 26 ] . Two adjacent sections stained by using our protocol and the manufacturer's recommended protocols were compared for specificity and sensitivity.


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