Example sentences for: deoxynucleoside

How can you use “deoxynucleoside” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).

  • For each PCR run, a master mixture was prepared on ice with 1× Platinum PCR buffer (Gibco/Life Technologies, Grand Island, NY, USA), 1.5 mM MgCl 2 , 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each primer, 2.5 units platinum Taq DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA).

  • For each gene, 5 μl of cDNA was incorporated into a 25 μl PCR mixture containing 8.8 mM TrisHCL, 44 mM KCl, 1.3 mM MgCl 2 , 0.00088 % gelatin, 0.284 mM each deoxynucleoside triphosphate, 1 U of Taq DNA polymerase (Applied Biosystems, Foster City, CA) and 1.78 μM of each primer.


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