Words similar to deoxynucleotidyl
Example sentences for: deoxynucleotidyl
How can you use “deoxynucleotidyl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method was used to demonstrate apoptosis in SMCs and endothelial cells [ 6 ] . The TUNEL was performed on frozen sections.
Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) (In situ Cell Death Detection Kit, POD, Boehringer-Manheim).
BSA = Bovine serum albumin; CSC = cigarette smoke condensate; ERK = extracellular signal-regulated kinase; GM-CSF = granulocyte-macrophage colony-stimulating factor; HBE = human bronchial epithelial cell; IL = interleukin; IκB = inhibitor of NFκB; MAPK = mitogen-activated protein kinase; MEK-1 = MAPK kinase; NFκB = nuclear factor-kappa B; NHBE = normal human bronchial epithelial cell; PBS = phosphate-buffered saline; PDTC = pyrrolidine dithiocarbamate; RT-PCR = reverse transcriptase-polymerase chain reaction; sICAM = soluble intercellular adhesion molecule; TNF = tumour necrosis factor; TUNEL = deoxynucleotidyl transferase-mediated dUTP nick end labeling
The induction of apoptosis was determined by means of the TUNEL (deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay using a kit supplied by Promega (Madison, WI, USA).
After washing twice with 1.0 ml PBS, the cells were resuspended in 50 μl of TdT buffer containing 0.2 M potassium cacodylate, 2.5 mM Tris-HCL (pH 6.6), 2.5 mM CoCl 2 , 0.25 mg/ml bovine serum albumin, 5.0 units of terminal deoxynucleotidyl transferase and 0.5 nM biotinylated deoxyuridine triphosphate (b-UTP) and incubated at 37°C for 30 minutes.
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