Words similar to deoxynucleotidyl
Example sentences for: deoxynucleotidyl
How can you use “deoxynucleotidyl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method was used to demonstrate apoptosis in SMCs and endothelial cells [ 6 ] . The TUNEL was performed on frozen sections.
The induction of apoptosis was determined by means of the TUNEL (deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay using a kit supplied by Promega (Madison, WI, USA).
BSA = Bovine serum albumin; CSC = cigarette smoke condensate; ERK = extracellular signal-regulated kinase; GM-CSF = granulocyte-macrophage colony-stimulating factor; HBE = human bronchial epithelial cell; IL = interleukin; IκB = inhibitor of NFκB; MAPK = mitogen-activated protein kinase; MEK-1 = MAPK kinase; NFκB = nuclear factor-kappa B; NHBE = normal human bronchial epithelial cell; PBS = phosphate-buffered saline; PDTC = pyrrolidine dithiocarbamate; RT-PCR = reverse transcriptase-polymerase chain reaction; sICAM = soluble intercellular adhesion molecule; TNF = tumour necrosis factor; TUNEL = deoxynucleotidyl transferase-mediated dUTP nick end labeling
After washing twice with 1.0 ml PBS, the cells were resuspended in 50 μl of TdT buffer containing 0.2 M potassium cacodylate, 2.5 mM Tris-HCL (pH 6.6), 2.5 mM CoCl 2 , 0.25 mg/ml bovine serum albumin, 5.0 units of terminal deoxynucleotidyl transferase and 0.5 nM biotinylated deoxyuridine triphosphate (b-UTP) and incubated at 37°C for 30 minutes.
Chondrocyte maturation was assessed on the basis of cell proliferation, cellular hypertrophy, alkaline phosphase activity and expression of Collagen type II and type X. Apoptosis was investigated by TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triposphate nick end labeling).