Words similar to deoxynucleotidyl
Example sentences for: deoxynucleotidyl
How can you use “deoxynucleotidyl” in a sentence? Here are some example sentences to help you improve your vocabulary:
BSA = Bovine serum albumin; CSC = cigarette smoke condensate; ERK = extracellular signal-regulated kinase; GM-CSF = granulocyte-macrophage colony-stimulating factor; HBE = human bronchial epithelial cell; IL = interleukin; IκB = inhibitor of NFκB; MAPK = mitogen-activated protein kinase; MEK-1 = MAPK kinase; NFκB = nuclear factor-kappa B; NHBE = normal human bronchial epithelial cell; PBS = phosphate-buffered saline; PDTC = pyrrolidine dithiocarbamate; RT-PCR = reverse transcriptase-polymerase chain reaction; sICAM = soluble intercellular adhesion molecule; TNF = tumour necrosis factor; TUNEL = deoxynucleotidyl transferase-mediated dUTP nick end labeling
In situ detection of DNA fragmentation was performed by incorporation of fluorescein-12-dUTP at the 3'-OH ends of DNA using Terminal deoxynucleotidyl Transferase (TUNEL assay) (Apoptosis Detection System, Fluorescein, Promega, Madison, WI), as performed previously by this laboratory [ 7 26 ] . Nuclei were counterstained using propidium iodide (Sigma Chemical Co., St. Louis, MO).
DNA fragmentation was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, using fluorescein-12-dUTP, according to the instructions supplied by the manufacturer (Promega Corp., Madison, WI).
After washing twice with 1.0 ml PBS, the cells were resuspended in 50 μl of TdT buffer containing 0.2 M potassium cacodylate, 2.5 mM Tris-HCL (pH 6.6), 2.5 mM CoCl 2 , 0.25 mg/ml bovine serum albumin, 5.0 units of terminal deoxynucleotidyl transferase and 0.5 nM biotinylated deoxyuridine triphosphate (b-UTP) and incubated at 37°C for 30 minutes.
Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method was used to demonstrate apoptosis in SMCs and endothelial cells [ 6 ] . The TUNEL was performed on frozen sections.
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