Example sentences for: deoxynucleotide

How can you use “deoxynucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The genes included: Rv3052c (probable nrdI ) which is postulated to be involved in deoxynucleotide production under stressed conditions; Rv0101 which is a nonribosomal peptide synthetase that displays strong homology with a Pseudomonas nonribosomal peptide synthetase required for the synthesis of the pyoveridine, a siderophore involved in iron uptake; Rv3420c which displays homology with the S18 ribosomal protein acetyltransferase which behaves as a heat shock protein in Chlamydia trachomatis; and Rv0497 and Rv3604c which are hypothetical conserved membrane proteins of unknown function.

  • PCR was performed with 200 ng genomic DNA, 50 μM each deoxynucleotide triphosphate, 02 μM of forward and reverse primers each and 2.5 units of Taq Polymerase (life Technologies).

  • Taq polymerase, deoxynucleotide triphosphates and Taq buffer were from Promega (Madison, WI, USA).

  • Each 100 μl PCR contained 3-5 μl of template DNA, 5 μl of dimethyl sulfoxide (DMSO), and 90-92 μl of a reaction mixture (200 μM (each) deoxynucleotide triphosphates, 1.0 μM (each) primer, 1.25 U of AmpliTaq DNA Polymerase (Perkin-Elmer Cetus), 10 mM Tris hydrochloride pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , and 0.01% (w/v) gelatin).

  • Each 10 μl PCR reaction mixture contained 1 × PCR buffer, 1.92 mM MgCl 2 , 0.25 units Taq DNA polymerase, 0.2 mM of each deoxynucleotide, 132 nM of the primers, and 50 ng genomic DNA.

How many words do you know? Try our free vocabulary size test!


Search for example sentences

Loading Loading...