Example sentences for: deoxynucleotide

How can you use “deoxynucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • dNTPs, deoxynucleotide triphosphates

  • PCR was performed with 200 ng genomic DNA, 50 μM each deoxynucleotide triphosphate, 02 μM of forward and reverse primers each and 2.5 units of Taq Polymerase (life Technologies).

  • The genes included: Rv3052c (probable nrdI ) which is postulated to be involved in deoxynucleotide production under stressed conditions; Rv0101 which is a nonribosomal peptide synthetase that displays strong homology with a Pseudomonas nonribosomal peptide synthetase required for the synthesis of the pyoveridine, a siderophore involved in iron uptake; Rv3420c which displays homology with the S18 ribosomal protein acetyltransferase which behaves as a heat shock protein in Chlamydia trachomatis; and Rv0497 and Rv3604c which are hypothetical conserved membrane proteins of unknown function.

  • 50 μl amplification reactions contained 100-200 ng DNA template and 45 μl of a reaction mix (200 μM each, deoxynucleotide triphosphates; 1.0 μM each primer; 1.25 U Taq polymerase; 10 mM Tris hydrochloride (pH 8.3); 50 mM KCl; 1.5 mM MgCl 2 ; 0.01% gelatin).

  • Taq polymerase, deoxynucleotide triphosphates and Taq buffer were from Promega (Madison, WI, USA).


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