Words similar to deoxynucleotide
- denzil
- denzin
- denzir
- deoband
- deodato
- deoxygenated
- deoxyglucose
- deoxyhemoglobin
- deoxynucleoside
- deoxynucleoside-
- deoxynucleotide
- deoxynucleotides
- deoxynucleotidyl
- deoxynucleotidyltransferase
- deoxynucleotidyltransferase-mediated
- deoxyoligonuclotide
- deoxyribonucleic
- deoxyuridine
- depardieu
- depart
- departed
Example sentences for: deoxynucleotide
How can you use “deoxynucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:
The genes included: Rv3052c (probable nrdI ) which is postulated to be involved in deoxynucleotide production under stressed conditions; Rv0101 which is a nonribosomal peptide synthetase that displays strong homology with a Pseudomonas nonribosomal peptide synthetase required for the synthesis of the pyoveridine, a siderophore involved in iron uptake; Rv3420c which displays homology with the S18 ribosomal protein acetyltransferase which behaves as a heat shock protein in Chlamydia trachomatis; and Rv0497 and Rv3604c which are hypothetical conserved membrane proteins of unknown function.
Each 100 μl PCR contained 3-5 μl of template DNA, 5 μl of dimethyl sulfoxide (DMSO), and 90-92 μl of a reaction mixture (200 μM (each) deoxynucleotide triphosphates, 1.0 μM (each) primer, 1.25 U of AmpliTaq DNA Polymerase (Perkin-Elmer Cetus), 10 mM Tris hydrochloride pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , and 0.01% (w/v) gelatin).
Each 10 μl PCR reaction mixture contained 1 × PCR buffer, 1.92 mM MgCl 2 , 0.25 units Taq DNA polymerase, 0.2 mM of each deoxynucleotide, 132 nM of the primers, and 50 ng genomic DNA.
50 μl amplification reactions contained 100-200 ng DNA template and 45 μl of a reaction mix (200 μM each, deoxynucleotide triphosphates; 1.0 μM each primer; 1.25 U Taq polymerase; 10 mM Tris hydrochloride (pH 8.3); 50 mM KCl; 1.5 mM MgCl 2 ; 0.01% gelatin).
PCR was performed with 200 ng genomic DNA, 50 μM each deoxynucleotide triphosphate, 02 μM of forward and reverse primers each and 2.5 units of Taq Polymerase (life Technologies).
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