Example sentences for: deoxynucleotide

How can you use “deoxynucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Fluorescently labeled cDNA was produced using a two-step procedure involving cDNA production from target RNA using a reverse transcriptase reaction incorporating aminoallyl-modified deoxynucleotide (aadUTP), followed by the second step involving chemical coupling of fluorescent dye (either Cy3 or Cy5) to the introduced amino moieties of the newly synthesized cDNA.

  • PCR was performed with 200 ng genomic DNA, 50 μM each deoxynucleotide triphosphate, 02 μM of forward and reverse primers each and 2.5 units of Taq Polymerase (life Technologies).

  • Taq polymerase, deoxynucleotide triphosphates and Taq buffer were from Promega (Madison, WI, USA).

  • dNTPs, deoxynucleotide triphosphates

  • 50 μl amplification reactions contained 100-200 ng DNA template and 45 μl of a reaction mix (200 μM each, deoxynucleotide triphosphates; 1.0 μM each primer; 1.25 U Taq polymerase; 10 mM Tris hydrochloride (pH 8.3); 50 mM KCl; 1.5 mM MgCl 2 ; 0.01% gelatin).


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