Words similar to deoxynucleotide
- denzil
- denzin
- denzir
- deoband
- deodato
- deoxygenated
- deoxyglucose
- deoxyhemoglobin
- deoxynucleoside
- deoxynucleoside-
- deoxynucleotide
- deoxynucleotides
- deoxynucleotidyl
- deoxynucleotidyltransferase
- deoxynucleotidyltransferase-mediated
- deoxyoligonuclotide
- deoxyribonucleic
- deoxyuridine
- depardieu
- depart
- departed
Example sentences for: deoxynucleotide
How can you use “deoxynucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:
Taq polymerase, deoxynucleotide triphosphates and Taq buffer were from Promega (Madison, WI, USA).
Each 10 μl PCR reaction mixture contained 1 × PCR buffer, 1.92 mM MgCl 2 , 0.25 units Taq DNA polymerase, 0.2 mM of each deoxynucleotide, 132 nM of the primers, and 50 ng genomic DNA.
Fluorescently labeled cDNA was produced using a two-step procedure involving cDNA production from target RNA using a reverse transcriptase reaction incorporating aminoallyl-modified deoxynucleotide (aadUTP), followed by the second step involving chemical coupling of fluorescent dye (either Cy3 or Cy5) to the introduced amino moieties of the newly synthesized cDNA.
Each 100 μl PCR contained 3-5 μl of template DNA, 5 μl of dimethyl sulfoxide (DMSO), and 90-92 μl of a reaction mixture (200 μM (each) deoxynucleotide triphosphates, 1.0 μM (each) primer, 1.25 U of AmpliTaq DNA Polymerase (Perkin-Elmer Cetus), 10 mM Tris hydrochloride pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , and 0.01% (w/v) gelatin).
PCR was performed with 200 ng genomic DNA, 50 μM each deoxynucleotide triphosphate, 02 μM of forward and reverse primers each and 2.5 units of Taq Polymerase (life Technologies).
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