Example sentences for: deoxynucleotide

How can you use “deoxynucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR was performed with 200 ng genomic DNA, 50 μM each deoxynucleotide triphosphate, 02 μM of forward and reverse primers each and 2.5 units of Taq Polymerase (life Technologies).

  • Each 100 μl PCR contained 3-5 μl of template DNA, 5 μl of dimethyl sulfoxide (DMSO), and 90-92 μl of a reaction mixture (200 μM (each) deoxynucleotide triphosphates, 1.0 μM (each) primer, 1.25 U of AmpliTaq DNA Polymerase (Perkin-Elmer Cetus), 10 mM Tris hydrochloride pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , and 0.01% (w/v) gelatin).

  • Fluorescently labeled cDNA was produced using a two-step procedure involving cDNA production from target RNA using a reverse transcriptase reaction incorporating aminoallyl-modified deoxynucleotide (aadUTP), followed by the second step involving chemical coupling of fluorescent dye (either Cy3 or Cy5) to the introduced amino moieties of the newly synthesized cDNA.

  • The genes included: Rv3052c (probable nrdI ) which is postulated to be involved in deoxynucleotide production under stressed conditions; Rv0101 which is a nonribosomal peptide synthetase that displays strong homology with a Pseudomonas nonribosomal peptide synthetase required for the synthesis of the pyoveridine, a siderophore involved in iron uptake; Rv3420c which displays homology with the S18 ribosomal protein acetyltransferase which behaves as a heat shock protein in Chlamydia trachomatis; and Rv0497 and Rv3604c which are hypothetical conserved membrane proteins of unknown function.

  • Taq polymerase, deoxynucleotide triphosphates and Taq buffer were from Promega (Madison, WI, USA).

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