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Example sentences for: deleted
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The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.
For most analyses, short parts of the receptor sequences corresponding to the extreme amino termini (upstream of the conserved predicted N -linked glycosylation site) and carboxyl termini (beyond 12 amino acids residues downstream of TM7) were deleted to avoid length heterogeneity in the compared sequences.
In each case, other sequences within extended ars3002 must be capable of providing at least a portion of the function of the deleted region.
The uvrB and uvrC genes fall in separate large deleted regions between syntenic fragments, whereas uvrA is one of six genes deleted within a syntenic region, with the corresponding position in Buchnera occupied by a 618 bp spacer (Table 1).
The PTEN gene is also frequently deleted or mutated in a variety of sporadic human malignancies, such as glioblastoma and carcinomas of the prostate, endometrium and breast.
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