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Example sentences for: dd
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A heuristic search without bootstrapping also showed the Dd myoI myosin as a branch from class VII and domain analysis shows that Dd myoI has the MyTH4 domain found in other class VII myosins.
P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
Under DD conditions, less REMS was present in cry1,2 -/-mice than in cry1,2 +/+mice (119 ± 7 vs. 149 ± 9 min, P < 0.03, unpaired t-test) due to an increase in REMS in cry1,2 +/+mice in DD (149 ± 9 min) relative to LD (113 ± 10 min, P < 0.03, paired t-test).
In order to investigate the endogenous rhythmicity of mNoc expression, C3H/He mice were maintained in constant darkness for 36 hours before sampling for rhythmic changes in DD.
Previous studies of cryptochrome's chronobiological role in Drosophila emphasized that CRY may function as a deep brain photoreceptor involved in mediating resetting of the clock by light [ 26 ] . Support for this hypothesis came from the observation that locomotor-activity rhythms in cry b flies are unaffected in constant darkness and also maintain rhythmicity under constant light, a condition that causes behavioral arrhythmicity in the wild type [ 33 ] . While antennal rhythms of gene expression and of odor sensitivity in DD are significantly attenuated in cry b tissues ( [ 23 ] , unlike locomotor rhythmicity [ 21 ] ), it remained possible that such effects are due to an altered role of light on the developing clock.
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