Words similar to dde
Example sentences for: dde
How can you use “dde” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 U Bfa I, 1.0 U Dde I, 1.5 U Dpn I, 2.0 U Alu I, 1.0 U Rsa I, or 2.0 U Hinf I) were diluted in 10 μl buffer (20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA) and added separately to the six rections (FAM: Bfa I and Dde I, JOE: Dpn I and Alu I, NED: Rsa I and Hinf I).
Contamination of the Great Lakes has led to the bioaccumulation of polychlorinated biphenyls (PCBs) in fish, particularly the larger predator species prized by sport-fishers [ 1 2 3 ] . These compounds are synthetic organochlorines (OC) that, along with dichlorodiphenyl-dichloroethene (DDE), comprise the bulk of OC residues found in human tissues [ 4 ] , and some of the highest body burdens of PCBs in humans are found among fish consumers [ 5 6 7 8 ] . Consumption of these contaminated Great Lakes fish has been associated with several adverse reproductive outcomes including shortened menstrual cycle [ 9 ] , reduced fecundability [ 10 11 ] , reduced neonatal size [ 12 ] , and neurologic disorders [ 13 14 15 ] .
After 2 hours at 37°C, the reactions were stopped by heating to 80°C for 20 minutes and the reactions Bfa I, Dpn I and Rsa I as well as Dde I, Alu I and Hinf I were pooled, respectively.
In our sample, this imputation procedure resulted in total PCB concentrations that were only marginally greater than those obtained if all nondetects were assigned 0. The mean PCB concentrations by exposure category are shown in table 3for both the sum using imputed values for nondetects and the sum with nondetects assigned 0. The limit of detection for DDE in the Wisconsin State Laboratory of Hygiene was 0.25 ng/mL.
Five milliliters of serum was analyzed for DDE and several PCB congeners using the capillary column gas chromatography with the electron capture method [ 37 ] . Copies of the PCB and DDE laboratory methods and quality control protocols are available from the authors upon request.
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