Words similar to cycloheximide
Example sentences for: cycloheximide
How can you use “cycloheximide” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 mM mgCl2, 10 mM Hepes, pH 6.8, 0.1 mM dithiothreitol, 10 ug/ml cycloheximide) and centrifuged at 4°C in a SW50.
The medium was then changed with readdition of pon A or an equal volume of ethanol and the protein synthesis inhibitor cycloheximide (100 μg/ml, Sigma) was added.
In the presence of cycloheximide, Sic1 accumulation was abolished, but Clb2 degradation still occurred with apparently normal kinetics (Figure 1, lanes 5-8 and 9-12).
5 mM mgCl2, 10 mM Hepes, pH 6.8, 0.1 mM dithiothreitol, 10 ug/ml cycloheximide, 0.5% Triton X-100, 0.5% deoxycholate) followed by 10 passages through a 22 gauge needle to lyse the cells.
Interestingly, Clb2 was rapidly and efficiently degraded upon reversal of the cdc15-2 block regardless of whether cycloheximide was present (Figure 1) although both cytokinesis and cell separation require protein synthesis [ 42 ] . This observation suggests that the exit from mitosis in budding yeast may be controlled by mechanisms fundamentally similar to those employed by animal cells, which proceed through mitosis with highly condensed, transcriptionally silent chromosomes.