Words similar to cycloheximide
Example sentences for: cycloheximide
How can you use “cycloheximide” in a sentence? Here are some example sentences to help you improve your vocabulary:
In addition, 10 μg/mL cycloheximide (CHX) was included to block protein synthesis, so that only immediate/early IL-2-regulated genes were detected.
Along these lines, when the aminogylcosides (kanamycin, hygromycin B, paromycin and neomycin) due to their ability to inhibit protein synthesis by affecting ribosomal fidelity, or puromycin because of its competing ability with tRNAs for binding on the large ribosomal subunit, or cycloheximide which inhibit the large ribosomal subunit by preventing ribosomal movement along the mRNA, were used in active HIV-1 infection, it was found that both cycloheximide and puromycin produced the greatest decrease in HIV-1 inhibition, presumably by inhibiting the large subunit of the ribosome [ 41 ] .
Uninduced and induced LLWO2F cells (10 × 100 mm dishes) were washed 3-times with ice-cold serum-free DMEM containing 10 ug/ml cycloheximide.
Cochran and Stiles were among the first to use differential cloning methods to identify PDGF-regulated genes in fibroblasts [ 26 ] . These investigators focused on the expression of immediate/early PDGF-stimulated genes, by activating the cells in the presence of inhibitors of protein synthesis such as cycloheximide, thereby preventing the expression of secondary genes.
The requirement for protein synthesis in Wnt signaling means that we cannot use cycloheximide sensitivity as a criterion to discriminate between primary and secondary targets.