Example sentences for: cycler

How can you use “cycler” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples underwent 30 cycles of amplification with Perkin-Elmer Taq polymerase in a PTC Thermal Cycler (MJ Research, MA) (94° for 40 sec, 57° for 1 min, 72° for 2 min).

  • PCR was performed using an iCycler iQ thermic cycler equipped with a real-time detection system (Bio-Rad, Hercules, California, United States) and a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, California, United States).

  • Amplifications were performed in a thermal cycler with an initial denaturing step of 5 minutes at 96°C, followed by 25 cycles, with each cycle consisting of 1 minute at 96°C, 50 seconds at 60°C, 50 seconds at 70°C, and a final step of 3 minutes at 72°C.

  • mRNA transcripts were quantified by the dual-labeled fluorogenic probe method for real-time PCR, using a Prism 7900 thermal cycler and sequence detector (Applied Biosystems, Foster City, California, United States).

  • To encode a PCR reaction using this method the protocol could describe the thermal cycling conditions and the make/model of the instrument, while the protocol application might give the sequences of the primers and the serial number of the thermal cycler.


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