Example sentences for: cycler

How can you use “cycler” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 60 seconds, 63°C for 90 seconds for annealing, and 72°C for 90 seconds for extension.

  • mRNA transcripts were quantified by the dual-labeled fluorogenic probe method for real-time PCR, using a Prism 7900 thermal cycler and sequence detector (Applied Biosystems, Foster City, California, United States).

  • Samples underwent 30 cycles of amplification with Perkin-Elmer Taq polymerase in a PTC Thermal Cycler (MJ Research, MA) (94° for 40 sec, 57° for 1 min, 72° for 2 min).

  • The reaction was cycled 44 times at 94°C for 1 min, 35°C for 1.5 min and 72°C for 1.5 min in a thermal cycler (Robocycler 40, Stratagene GmbH, Germany).

  • Thermal cycler parameters included 2 min at 50°C, 10 min at 95°C, and 40 cycles involving denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min.


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