Words similar to cycler
Example sentences for: cycler
How can you use “cycler” in a sentence? Here are some example sentences to help you improve your vocabulary:
mRNA transcripts were quantified by the dual-labeled fluorogenic probe method for real-time PCR, using a Prism 7900 thermal cycler and sequence detector (Applied Biosystems, Foster City, California, United States).
We conducted the PCR reaction in a MJ Research (Watertown, Massachusetts) PTC-200 Peltier Thermal Cycler programmed with an initial cDNA synthesis and pre-denaturation step of 50°C for 30 minutes and 94°C for 2 minutes followed by then 36 cycles of denature, annealing and extension (15 s at 94°C, 30 s at 55°C, 30 s at 68°C).
Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 60 seconds, 63°C for 90 seconds for annealing, and 72°C for 90 seconds for extension.
Amplifications were performed in a thermal cycler with an initial denaturing step of 5 minutes at 96°C, followed by 25 cycles, with each cycle consisting of 1 minute at 96°C, 50 seconds at 60°C, 50 seconds at 70°C, and a final step of 3 minutes at 72°C.
To encode a PCR reaction using this method the protocol could describe the thermal cycling conditions and the make/model of the instrument, while the protocol application might give the sequences of the primers and the serial number of the thermal cycler.
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