Words similar to cycler
Example sentences for: cycler
How can you use “cycler” in a sentence? Here are some example sentences to help you improve your vocabulary:
Amplifications were performed in a thermal cycler with an initial denaturing step of 5 minutes at 96°C, followed by 25 cycles, with each cycle consisting of 1 minute at 96°C, 50 seconds at 60°C, 50 seconds at 70°C, and a final step of 3 minutes at 72°C.
PCR reactions were carried out in 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl 2 , 0.2 mM dNTPs and 1 μM primers at 94°C for 0.5 min, 60°C for 0.5 min, and 72°C for 1.0 min for 35 cycles in a Perkin Elmer Cetus 9600 thermal cycler.
PCR was performed using an iCycler iQ thermic cycler equipped with a real-time detection system (Bio-Rad, Hercules, California, United States) and a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, California, United States).
Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 60 seconds, 63°C for 90 seconds for annealing, and 72°C for 90 seconds for extension.
To encode a PCR reaction using this method the protocol could describe the thermal cycling conditions and the make/model of the instrument, while the protocol application might give the sequences of the primers and the serial number of the thermal cycler.