Example sentences for: cycled

How can you use “cycled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR reactions were cycled 35 times under the following conditions: 94 degrees for 30 seconds, 55 degrees for 1 minute and 72 degrees for 2 minutes.

  • 15 pmol of nt 1932 to nt 1959 primer and 15 pmol of nt 3100 to 3081 primer were added and further cycled for 4 minutes at 95°C, then 30X of 2 minutes at 95°C, 1 minute at 60°C, 1 minute at 72°C and finally 10 minutes at 72°C.

  • The reactions were carried out in 50 μl using 5 pmol of each primer and cycled as follows: denaturation at 94°C for 45 s; annealing at 65°C for 45 s; and extension at 72°C for 45 s.

  • The reactions were carried out in 25 μ l and cycled as follows: denaturation at 94°C for 30 s; annealing at 52°C for 45 s; and extension at 72°C for 30 s.

  • The agarose plug was added to a fresh PCR reaction (100 μl) and cycled 15 times; DNA was purified using a QIAquick kit and quantitated.


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