Example sentences for: cycled

How can you use “cycled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR reactions were cycled 35 times under the following conditions: 94 degrees for 30 seconds, 55 degrees for 1 minute and 72 degrees for 2 minutes.

  • The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 μl reactions under the conditions stated above and cycled 20 times.

  • The agarose plug was added to a fresh PCR reaction (100 μl) and cycled 15 times; DNA was purified using a QIAquick kit and quantitated.

  • These reactions were carried out in 50 μ l using 5 pmol of each primer, and were cycled with a 9600 GeneAmp System (Perkin Elmer, Emeryville, CA, USA) as follows: denaturation at 94°C for 40 s; annealing at 65°C for 45 s; and extension at 72°C for 40 s.

  • The reaction was cycled 44 times at 94°C for 1 min, 35°C for 1.5 min and 72°C for 1.5 min in a thermal cycler (Robocycler 40, Stratagene GmbH, Germany).


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