Example sentences for: cycled

How can you use “cycled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These reactions were carried out in 50 μ l using 5 pmol of each primer, and were cycled with a 9600 GeneAmp System (Perkin Elmer, Emeryville, CA, USA) as follows: denaturation at 94°C for 40 s; annealing at 65°C for 45 s; and extension at 72°C for 40 s.

  • The reactions were carried out in 25 μ l and cycled as follows: denaturation at 94°C for 30 s; annealing at 52°C for 45 s; and extension at 72°C for 30 s.

  • The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 μl reactions under the conditions stated above and cycled 20 times.

  • 15 pmol of nt 1932 to nt 1959 primer and 15 pmol of nt 3100 to 3081 primer were added and further cycled for 4 minutes at 95°C, then 30X of 2 minutes at 95°C, 1 minute at 60°C, 1 minute at 72°C and finally 10 minutes at 72°C.

  • The reactions were carried out in 50 μl using 5 pmol of each primer and cycled as follows: denaturation at 94°C for 45 s; annealing at 65°C for 45 s; and extension at 72°C for 45 s.


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