Example sentences for: cycled

How can you use “cycled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The agarose plug was added to a fresh PCR reaction (100 μl) and cycled 15 times; DNA was purified using a QIAquick kit and quantitated.

  • PCR reactions were cycled 35 times under the following conditions: 94 degrees for 30 seconds, 55 degrees for 1 minute and 72 degrees for 2 minutes.

  • The reaction was cycled 44 times at 94°C for 1 min, 35°C for 1.5 min and 72°C for 1.5 min in a thermal cycler (Robocycler 40, Stratagene GmbH, Germany).

  • The reactions were carried out in 50 μl using 5 pmol of each primer and cycled as follows: denaturation at 94°C for 45 s; annealing at 65°C for 45 s; and extension at 72°C for 45 s.

  • I also recall with affection the late John Leaver, who cycled around the English countryside as a boy collecting pub-names.


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