Example sentences for: cycled

How can you use “cycled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The agarose plug was added to a fresh PCR reaction (100 μl) and cycled 15 times; DNA was purified using a QIAquick kit and quantitated.

  • The reactions were carried out in 50 μl using 5 pmol of each primer and cycled as follows: denaturation at 94°C for 45 s; annealing at 65°C for 45 s; and extension at 72°C for 45 s.

  • I also recall with affection the late John Leaver, who cycled around the English countryside as a boy collecting pub-names.

  • PCR reactions were cycled 35 times under the following conditions: 94 degrees for 30 seconds, 55 degrees for 1 minute and 72 degrees for 2 minutes.

  • The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 μl reactions under the conditions stated above and cycled 20 times.


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