Words similar to cycle-regulatory
Example sentences for: cycle-regulatory
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A model to study cell regulatory events during anchorage-independent growth is the culture of cells in suspension, i.e. on HEMA-coated plates that prevent cells from attachment to the matrix of the cell culture dish [ 37 ] . Several studies have employed this approach and characterized the regulation of various cell cycle-regulatory proteins after the transfer of cells to such suspension culture [ 27 28 29 30 31 32 ] . Here, we have used this model to analyze the potential involvement of the tumor suppressor PTEN.
Total cell lysates were prepared by lysis of cells with RIPA buffer [ 63 ] . Thirty μg of each sample was processed by Western blot analysis as described [ 31 ] . Antibodies against cell cycle-regulatory proteins as well as those against focal adhesion kinase were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).