Words similar to ctp

Example sentences for: ctp

How can you use “ctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

The reaction buffer contained 10 mM dithiothreitol (DTT), 0.5 μM of ATP, CTP, and GTP, 2.5 μM of UTP, and 5 μl of 800 Ci/mmol [α- 32P] UTP at 10 mCi/ml (Dupont NEN).
Northern blot analysis was carried out using double-stranded DNA probes labeled to a specific activity of 10 8- 10 9cpm/μg with α-[ 32P] CTP using T7 DNA polymerase and random primers.
α- 32P CTP (800 Ci/mmol) and γ- 32P ATP (3000 Ci/mmol) were obtained from New England Nuclear (Boston, Ma).
Briefly, probes were prepared as follows: 1 μg of pGEM1/cCAF cDNA was transcribed for 1 1/2 h at 37°C in a cocktail containing 100 μM each of 3H-ATP and 3H-UTP (Amersham Corp.), 1 mM each GTP and CTP, 120 mM dithiothreitol, and 100 U of T7 polymerase (Amersham Corop.)
Commercially available 2-aminoadenosine-5'-triphosphate, 5-methylcytidine-5'-triphosphate, and 5-methyluridine-5'-triphosphate (TriLink Biotechnologies, Inc., San Diego, CA) were substituted for ATP, CTP, and UTP, respectively, in separate reactions containing either complete substitution, 1:1, or 1:3 ratio of modified: unmodified NTP, keeping molar input of nucleotide constant.