Words similar to ctp

Example sentences for: ctp

How can you use “ctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

A 499 bp nmt55/p54 nrbfragment was transcribed with T7 RNA polymerase in the presence of α-[ 32P] CTP and non-radioactive nucleotides to generate an antisense probe (specific activity of 10 8- 10 9cpm/μg) or SP6 RNA polymerase in the presence of non-labeled nucleotides to generate a sense probe (control).
Finally, line PdL(3)8R128 had an insert at the 5' end of the CTP:phosphocholine cytidylyltransferase 1 ( Cct1 ) gene (Figure 3f).
45 mm), and 32p-dCTP were obtained from Amersham (Arlington Heights, IL).
Briefly, probes were prepared as follows: 1 μg of pGEM1/cCAF cDNA was transcribed for 1 1/2 h at 37°C in a cocktail containing 100 μM each of 3H-ATP and 3H-UTP (Amersham Corp.), 1 mM each GTP and CTP, 120 mM dithiothreitol, and 100 U of T7 polymerase (Amersham Corop.)
The cDNA was purified using a Qiagen QIAquick purification kit, dried down, and resuspended in IVT reaction mix, containing 3.0 μl nuclease-free water, 4.0 μl 10X reaction buffer, 4.0 μl 75 mM ATP, 4.0 μl 75 mM GTP 3.0 μl 75 mM CTP, 3.0 μl 75 mM UTP, 7.5 μl 10 mM Biotin-11-CTP, 7.5 μl 10 mM Biotin 11-UTP and 4.0 μl enzyme mix (unmodified control condition).