Words similar to ctp

Example sentences for: ctp

How can you use “ctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
Finally, line PdL(3)8R128 had an insert at the 5' end of the CTP:phosphocholine cytidylyltransferase 1 ( Cct1 ) gene (Figure 3f).
Biotin labeled CTP and UTP (Enzo) was used together with unlabeled NTP's in the reaction.
The cDNA probe was prepared by restriction digestion of pET-GST-PPT1 with Bsm I and Nco I. The 879 bp DNA fragment generated, corresponding to 544-1422 of PPT1 coding sequence, was gel-purified and labeled with 0.67 μM [α- 32P] CTP (3000 Ci/mmol) using the DECAprimeII random priming kit (Ambion) according to the user manual.
5 mM), CTP (7.