Words similar to ctp

Example sentences for: ctp

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The transcription reaction mixtures contained lug of linearized DNA; 0.5 mM(each) ATP, CTP, and UTP; 0.1 mM GTP; 0.5 mM cap analog (NEBL); 10 mM DTT; 40 U of Rnasin (Promega); 30 U of SP6 RNA polymerase; and 1 × SP6 RNA polymerase buffer (Promega) in a volume of 30 ul.
Biotinylated CTP and UTP (Enzo Biochem Inc., NY) were used together with unlabeled dNTPs in the reaction, and unincorporated nucleotides were removed with RNeasy columns.
A 499 bp nmt55/p54 nrbfragment was transcribed with T7 RNA polymerase in the presence of α-[ 32P] CTP and non-radioactive nucleotides to generate an antisense probe (specific activity of 10 8- 10 9cpm/μg) or SP6 RNA polymerase in the presence of non-labeled nucleotides to generate a sense probe (control).
Northern blot analysis was carried out using double-stranded DNA probes labeled to a specific activity of 10 8- 10 9cpm/μg with α-[ 32P] CTP using T7 DNA polymerase and random primers.
α- 32P CTP (800 Ci/mmol) and γ- 32P ATP (3000 Ci/mmol) were obtained from New England Nuclear (Boston, Ma).