Words similar to ctp
Example sentences for: ctp
How can you use “ctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, probes were prepared as follows: 1 μg of pGEM1/cCAF cDNA was transcribed for 1 1/2 h at 37°C in a cocktail containing 100 μM each of 3H-ATP and 3H-UTP (Amersham Corp.), 1 mM each GTP and CTP, 120 mM dithiothreitol, and 100 U of T7 polymerase (Amersham Corop.)
α- 32P CTP (800 Ci/mmol) and γ- 32P ATP (3000 Ci/mmol) were obtained from New England Nuclear (Boston, Ma).
Poly(A) +cDNA was transcribed in vitro by incorporation of biotinylated CTP and UTP (Enzo Diagnostics, Farmingdale, NY) using a BioArray High Yield RNA Transcript Labeling kit according to the manufacturer's instructions.
The cDNA probe was prepared by restriction digestion of pET-GST-PPT1 with Bsm I and Nco I. The 879 bp DNA fragment generated, corresponding to 544-1422 of PPT1 coding sequence, was gel-purified and labeled with 0.67 μM [α- 32P] CTP (3000 Ci/mmol) using the DECAprimeII random priming kit (Ambion) according to the user manual.
Unlabeled UTP was present at 3.75 mM, while GTP, ATP, and CTP were at 5 mM.