Words similar to ctp
Example sentences for: ctp
How can you use “ctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
α- 32P CTP (800 Ci/mmol) and γ- 32P ATP (3000 Ci/mmol) were obtained from New England Nuclear (Boston, Ma).
One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).
Commercially available 2-aminoadenosine-5'-triphosphate, 5-methylcytidine-5'-triphosphate, and 5-methyluridine-5'-triphosphate (TriLink Biotechnologies, Inc., San Diego, CA) were substituted for ATP, CTP, and UTP, respectively, in separate reactions containing either complete substitution, 1:1, or 1:3 ratio of modified: unmodified NTP, keeping molar input of nucleotide constant.
Briefly, probes were prepared as follows: 1 μg of pGEM1/cCAF cDNA was transcribed for 1 1/2 h at 37°C in a cocktail containing 100 μM each of 3H-ATP and 3H-UTP (Amersham Corp.), 1 mM each GTP and CTP, 120 mM dithiothreitol, and 100 U of T7 polymerase (Amersham Corop.)
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