Words similar to ctp
Example sentences for: ctp
How can you use “ctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Unlabeled UTP was present at 3.75 mM, while GTP, ATP, and CTP were at 5 mM.
Northern blot analysis was carried out using double-stranded DNA probes labeled to a specific activity of 10 8- 10 9cpm/μg with α-[ 32P] CTP using T7 DNA polymerase and random primers.
The reaction buffer contained 10 mM dithiothreitol (DTT), 0.5 μM of ATP, CTP, and GTP, 2.5 μM of UTP, and 5 μl of 800 Ci/mmol [α- 32P] UTP at 10 mCi/ml (Dupont NEN).
CTP:phosphocholine cytidylyltransferase (Cct) catalyzes the conversion of phosphocholine to CDP-choline and is the rate-limiting enzyme in the phosphatidylcholine biosynthetic pathway [ 41].
One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
Loading...