Words similar to ctp

Example sentences for: ctp

How can you use “ctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
The cDNA was purified using a Qiagen QIAquick purification kit, dried down, and resuspended in IVT reaction mix, containing 3.0 μl nuclease-free water, 4.0 μl 10X reaction buffer, 4.0 μl 75 mM ATP, 4.0 μl 75 mM GTP 3.0 μl 75 mM CTP, 3.0 μl 75 mM UTP, 7.5 μl 10 mM Biotin-11-CTP, 7.5 μl 10 mM Biotin 11-UTP and 4.0 μl enzyme mix (unmodified control condition).
Poly(A) +cDNA was transcribed in vitro by incorporation of biotinylated CTP and UTP (Enzo Diagnostics, Farmingdale, NY) using a BioArray High Yield RNA Transcript Labeling kit according to the manufacturer's instructions.
The reaction buffer contained 10 mM dithiothreitol (DTT), 0.5 μM of ATP, CTP, and GTP, 2.5 μM of UTP, and 5 μl of 800 Ci/mmol [α- 32P] UTP at 10 mCi/ml (Dupont NEN).
α- 32P CTP (800 Ci/mmol) and γ- 32P ATP (3000 Ci/mmol) were obtained from New England Nuclear (Boston, Ma).