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Example sentences for: crnas

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The spiked transcripts and their final concentrations in the hybridization cocktails are listed in Table 1. Final concentrations in pmol and parts per million (ppm) for each spiked transcript were computed from the known length of each spike-in, assuming a total mass of 2 μg worm cRNA in a 200 μl hybridization volume, and an average length of 1,000 bases for in vitro transcribed worm cRNAs.
F snormalization is based on the principle of removing technical variation in the ratio of spiked transcripts to cDNA-template-derived cRNAs, by averaging the response to spiked cRNAs over multiple hybridizations.
Each of the 10 plasmids was used to synthesize cRNA in vitro . cRNAs were quantitated and differentially spiked into heart and brain RNA samples at specific copy numbers based on the following assumptions: 360,000 mRNA transcripts per cell, 20 pg total RNA per cell and 1 pg mRNA transcript per cell; hence, 100 'spike' copies/cell would be equivalent to about 0.14 ng 'spike' transcript in 10 μg total RNA [ 12].
987) was higher than those observed with multiple cRNAs from the same total RNA source (0.
To normalize our array data, we spiked five of the ten control cRNAs into the Cy3 and Cy5 labeling reactions at equal copy number, ranging from 40 to 100 copies per cell.