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Example sentences for: crnas

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F snormalization is based on the principle of removing technical variation in the ratio of spiked transcripts to cDNA-template-derived cRNAs, by averaging the response to spiked cRNAs over multiple hybridizations.
A calibration function is then computed by fitting a single linear model to the scaled average differences of all spiked cRNAs on all the arrays in the dataset, pooled together.
The resulting profiles were also compared to previously reported mixed 6-9 yr old DMD patient cRNAs, and mixed 6-9 yr old control cRNAs [ 23 ] , as mentioned above.
The spike-skew factor for the spiked cRNAs was Gaussian with mean 1 and a SD that was adjusted from 0.1 to 0.4 (in percentage terms, 10-40%).
After hybridization and washing, arrays were stained with phycoerythrin-streptavidin, which binds to the biotinylated cRNAs hybridized with the probes.