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Example sentences for: crnas

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Our results suggest that there is significant uncertainty in the molar ratio of spike-in mRNAs to template-derived cRNAs in any hybridization (the spike-skew effect).
A calibration function is then computed by fitting a single linear model to the scaled average differences of all spiked cRNAs on all the arrays in the dataset, pooled together.
The spike-skew factor for the spiked cRNAs was Gaussian with mean 1 and a SD that was adjusted from 0.1 to 0.4 (in percentage terms, 10-40%).
The spiked transcripts and their final concentrations in the hybridization cocktails are listed in Table 1. Final concentrations in pmol and parts per million (ppm) for each spiked transcript were computed from the known length of each spike-in, assuming a total mass of 2 μg worm cRNA in a 200 μl hybridization volume, and an average length of 1,000 bases for in vitro transcribed worm cRNAs.
In other words, F svalues are estimates of transcript abundance in cRNA, based on the average response to the spiked cRNAs over multiple hybridizations and on the sensitivity of each individual array.