Words similar to crnas

Example sentences for: crnas

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To generate cRNAs containing a 3' poly(A) tail, pSP64poly(A) constructs were linearized with Eco RI and in vitro transcribed from the SP6 promoter using the MEGAscript™ High Yield Transcription Kit (Ambion, Austin, TX).
The intensity of the hybridization signals were determined as previously reported [ 26 27 28 ] . A subjective comparison of eight sections from each ovary from six animals hybridized with the same concentrations of sense and antisense cRNAs and exposed for the same time (4 weeks) was made.
Even at a printing concentration as low as 6 μM, oligonucleotide probes were capable of discerning twofold expression differences in complex cellular RNA mixtures and in synthetic spiked cRNAs.
The cRNAs were then hybridized to the MuscleChip either singly, in mixed groups, or both, as described below.
In other words, F svalues are estimates of transcript abundance in cRNA, based on the average response to the spiked cRNAs over multiple hybridizations and on the sensitivity of each individual array.