Words similar to crnas
Example sentences for: crnas
How can you use “crnas” in a sentence? Here are some example sentences to help you improve your vocabulary:
F values provide the same estimate, but based solely on the response to spiked cRNAs in a single array hybridization.
Each of the 10 plasmids was used to synthesize cRNA in vitro . cRNAs were quantitated and differentially spiked into heart and brain RNA samples at specific copy numbers based on the following assumptions: 360,000 mRNA transcripts per cell, 20 pg total RNA per cell and 1 pg mRNA transcript per cell; hence, 100 'spike' copies/cell would be equivalent to about 0.14 ng 'spike' transcript in 10 μg total RNA [ 12].
Arabidopsis spiking control cRNAs along with their corresponding 70-mer oligonucleotide and PCR amplicon probes.
Our results suggest that there is significant uncertainty in the molar ratio of spike-in mRNAs to template-derived cRNAs in any hybridization (the spike-skew effect).
To generate cRNAs containing a 3' poly(A) tail, pSP64poly(A) constructs were linearized with Eco RI and in vitro transcribed from the SP6 promoter using the MEGAscript™ High Yield Transcription Kit (Ambion, Austin, TX).
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