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Example sentences for: crnas

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We have shown that cRNAs spiked into hybridization solutions at known concentrations covering two to three orders of magnitude can be used to normalize array data and to estimate array sensitivity.
The spiked transcripts and their final concentrations in the hybridization cocktails are listed in Table 1. Final concentrations in pmol and parts per million (ppm) for each spiked transcript were computed from the known length of each spike-in, assuming a total mass of 2 μg worm cRNA in a 200 μl hybridization volume, and an average length of 1,000 bases for in vitro transcribed worm cRNAs.
To test for the relative sensitivity of interpretation of sample mixing versus individual profiles, we mixed together the 10 cRNAs for the two different age groups of DMD patients (samples 1a - 5b; samples 6a - 10b).
If there was no technical variation in the ratio of spiked transcripts to cDNA-template-derived cRNAs in the ten experiments, both approaches would give the same quantitation, up to a random error term arising from the difference between fitting ten 11-point responses versus a single 121-point response.
If, in one of the ten arrays, the ratio of spiked transcripts to cDNA-template-derived cRNAs is different for technical reasons, then spike response for that array will be skewed, and the F-metric readout for that array will be skewed relative to the other nine arrays.