Words similar to cpla

Example sentences for: cpla

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Diminution of AA release by MEK1/ERK is also independent of cPLA 2 phosphorylation on Ser 505.
Translocation to membrane is a critical regulatory step for the action of cPLA 2 because it is necessary for access to substrate.
2 , it is necessary to over-express the enzyme several fold as previously reported [ 16 ] . Cytosolic PLA 2 has been shown to mediate Ca 2+-induced AA release in MDCK cells treated with ATP and IONO in experiments using the group IV cPLA 2 α-specific inhibitor pyrrolidine-1 [ 27 ] . To measure cPLA 2 mediated AA release, EGFP-cPLA 2 -transfected MDCK cells labeled with [ 3H]-AA were incubated with 0.3, 1 or 10 μM U0126 for 15 min prior to stimulation with 100 μM ATP, 1 μM IONO, or 10 μM IONO.
MDCK cells were co-transfected with EYFP-cPLA 2 and a cPLA 2 with a S505A mutation fused to ECFP (ECFP-cPLA 2 S505A).
However, MEK inhibition only partially affected cPLA 2 phosphorylation and had no effect on the kinetics of Ca 2+-mediated cPLA 2 translocation to membrane.