Example sentences for: cpla
How can you use “cpla” in a sentence? Here are some example sentences to help you improve your vocabulary:
We have previously reported that p38 is also selectively activated in anisomycin-treated macrophages [ 21 ] . Importantly, pretreatment of cells for 30 min with anisomycin resulted in maintenance of the cPLA 2 gel shift in the presence of U0126 in unstimulated cells and in cells stimulated with ATP and 1 and 10 μM IONO (Fig.
However, MEK inhibition only partially affected cPLA 2 phosphorylation and had no effect on the kinetics of Ca 2+-mediated cPLA 2 translocation to membrane.
An amino terminal, calcium-dependent lipid binding (CaLB or C2) domain regulates Ca 2+-mediated cPLA 2 translocation to intracellular membranes [ 9 ] . In vitro, membrane docking via the C2 domain is necessary and sufficient for catalysis and release of AA [ 10 ] . Binding of calcium ions by the cPLA 2 C2 domain is essential for the lipid association in vitro [ 11 12 ] and translocation in vivo [ 13 14 ] . In response to an increase in [Ca 2+] i , cPLA 2 translocates to the Golgi and ER, however translocation to Golgi occurs at a lower [Ca 2+] i [ 15 ] .
1 cells, without constitutively active ERKs, AA release is delayed after [Ca 2+] i mobilization, and is temporally correlated with ERK activation [ 40 ] , whereas in MDCK cells with constitutively active ERKs, AA release is rapid, with significant AA release measured 30 s after [Ca 2+] i increase [ 15 ] . This temporal correlation between AA release and ERK activation has also been reported in CHO cells in response to PAF stimulation [ 35 41 ] . These results support the observations made here that ERK activity is required for phospholipid hydrolysis independently of cPLA 2 translocation.
Due to the increased molecular weight of the EGFP-tagged cPLA 2 , the two forms of cPLA 2 did not separate as well, making the gel shift more difficult to visualize, but generally mirrored the gel shift characteristics of the endogenous cPLA 2 . These results suggest that, in response to MEK1 inhibition, there is a quantitative, dose-dependent decrease in AA release that correlates well with the loss of ERK activation, but not with the extent of cPLA 2 Ser 505phosphorylation.
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