Words similar to crna
Example sentences for: crna
How can you use “crna” in a sentence? Here are some example sentences to help you improve your vocabulary:
Detailed procedures for preparation of cDNA and fluorescently labeled cRNA, hybridization, staining, and scanning of gene chips and assay monitoring are outlined in earlier literature [ 15 ] . The platform chosen for global expression profile was the Rat Genome U34 Array (Affymetrix, Santa Clara, California), which contains probes for a total of 8799 expressed sequence tag (EST) clusters and genes (including controls).
The biotinylated cRNA is hybridized to the GeneChip ®, unhybridized material is washed off, and the signal is detected using fluorescein-labeled Streptavidin [ 8 ] .
Total RNA (20 μg) was used to synthesize double-stranded cDNA using Superscript ®Choice System (Life Technologies), and the template for an in vitro transcription reaction was used to synthesize biotin-labeled antisense cRNA (BioArray™ High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA).
In the absence of cRNA spiking, cross-hybridization of Cy-labeled rat targets to
Mills and Gordon recently studied the relative contribution of experimental variability of probe production on the reproducibility of microarray results using mixed murine tissue RNA on Affymetrix Mu11K GeneChips [ 20 ] . In their study, the same RNA preparation was used as a template for distinct cDNA/cRNA amplifications and hybridizations.