Words similar to crna

Example sentences for: crna

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When the CVs in the ratios generated using either the control or partially modified cRNA are binned according to the magnitude of the CV, the partially modified cRNA generates more CVs in the less than 20% bins (Figure 6C).
To address these questions, we used an analytical method developed in our laboratory and described in a previous study [ 24 ] . Briefly, the complex cRNA is digested with P1 nuclease and calf intestinal phosphatase and applied to a high performance liquid chromatography (HPLC) column to separate the nucleosides, followed by an absorbance measurement at 260 nm.
The use of approximately 1 μg cRNA in each hybridization, or reduced scanner gain, largely eliminated saturation with no penalty in sensitivity.
Therefore, mass input of cRNA target samples were much greater for unmodified samples compared to the DAP-containing samples.
The standard protocol uses 10 μg of total RNA to produce biotinylated cRNA [ 8 ] . In our experiment, the average yield of biotinylated cRNA was 97 μg (± 41 μg; mean ± standard deviation) when we started with 10 μg of RNA and used one-half of the double stranded cDNA product in the in vitro transcription reaction.