Words similar to crna
Example sentences for: crna
How can you use “crna” in a sentence? Here are some example sentences to help you improve your vocabulary:
In addition, a recent publication of a single human patient, where RNA was prepared from two distinct breast tumors, and placed on duplicate U95A GeneChips (four chips total) found a very low degree of experimental variability between microarrays (R 2= 0.995), and between the two tumors (R 2= 0.987) [ 21 ] . The marked differences in experimental variability between laboratories could be due to different quality control protocols (see http://microarray.cnmcresearch.org), newer more robust Affymetrix arrays now available (murine Mu11K versus U74A version 2 and new generation human U95 series), use of more recent algorithms for data interpretation, or due to more consistent processing of RNA, cDNA, and cRNA in the same laboratory.
Labeled cRNA was hybridized on the HuGeneFL Affymetrix GeneChip ®containing probes for approximately 5600 mRNAs corresponding to genes of known sequence.
In order to measure the variation in gene expression between individual Affymetrix oligonucleotide arrays, the labeled cRNA target prepared from tumors 5A and 5B were each hybridized to two different U95A chips.
An early study on DNA microarray hybridization found that hybridization was strongly dependent on the rate constants for DNA adsorption/desorption in the non-probe covered regions of the surface, the two-dimensional diffusion coefficient, and the size of probes and targets and also suggested that sparse probe coverage may provide results equal to or better than those obtained with a surface totally covered with DNA probes [ 19 ] . A theoretical analysis of the kinetics of DNA hybridization demonstrated that diffusion was important in determining the time of required to reach equilibrium and was proportional to the equilibrium binding constant and to the concentration of binding sites [ 20 ] . A recent theoretical and experimental analysis of competitive hybridization in a two-color system demonstrated the need for the hybridization kinetics of the two probes to be the same [ 21 ] . An elegant study on the use of hybridization kinetics to differentiate specific from non-specific binding was recently published [ 22 ] . This study found that the hybridization kinetics for specific and non-specific binding of labeled cRNA to surface-bound oligodeoxyribonucleotides were significantly different, with specific binding requiring longer to reach hybridization equilibrium than non-specific binding.
the same biotinylated cRNA on two different microarrays).
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