Words similar to crna
Example sentences for: crna
How can you use “crna” in a sentence? Here are some example sentences to help you improve your vocabulary:
Labeled cRNA was prepared using the MEGAscript, In Vitro Transcription kit (Ambion).
In the control situation (hybridization of unmodified cRNA), the hybridization intensity decreased as the number of mismatches increased, with two mismatches generally destabilizing the duplex sufficiently to reduce the hybridization signal to 0% of the parent signal.
In a fourth probe sequence, the fully modified cRNA showed a dramatic decrease in specificity with one- or two-base mismatches, although three mismatches reduced the hybridization intensity to ~10% of that of the perfect match.
Biotinylated cRNA was prepared using the standard Affymetrix protocol [ 8 ] except that the Epicentre AmpliScribe™ T7 polymerase (Epicentre, Madison, WI) was substituted for the ENZO T7 polymerase (BioArray, High Yield RNA Transcript Labeling Kit, ENZO Diagnostics, Inc., Farmingdale, NY) for 2 of the treated and 2 of the untreated pooled samples.
The intensities of these probe sets was measured after different array hybridization times to generate the data shown in Figure 3. Lastly, the probes on the Human Uniset I arrays which were designed to measure the endogenous human transcripts in polyA+ RNA were measured after different array hybridization times and with different amounts of labeled cRNA to generate the data shown in Figure 4A.
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