Example sentences for: crna

How can you use “crna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Detailed procedures for preparation of cDNA and fluorescently labeled cRNA, hybridization, staining, and scanning of gene chips and assay monitoring are outlined in earlier literature [ 15 ] . The platform chosen for global expression profile was the Rat Genome U34 Array (Affymetrix, Santa Clara, California), which contains probes for a total of 8799 expressed sequence tag (EST) clusters and genes (including controls).

  • The biotinylated cRNA is hybridized to the GeneChip ®, unhybridized material is washed off, and the signal is detected using fluorescein-labeled Streptavidin [ 8 ] .

  • Total RNA (20 μg) was used to synthesize double-stranded cDNA using Superscript ®Choice System (Life Technologies), and the template for an in vitro transcription reaction was used to synthesize biotin-labeled antisense cRNA (BioArray™ High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA).

  • In the absence of cRNA spiking, cross-hybridization of Cy-labeled rat targets to

  • Mills and Gordon recently studied the relative contribution of experimental variability of probe production on the reproducibility of microarray results using mixed murine tissue RNA on Affymetrix Mu11K GeneChips [ 20 ] . In their study, the same RNA preparation was used as a template for distinct cDNA/cRNA amplifications and hybridizations.


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