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Example sentences for: cre

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Figure 3Ashows an experiment in which the R26R-YFP mice were crossed with the Isl1-Cre mice, resulting in expression of EYFP in motor neurons and dorsal root ganglia in the doubly transgenic offspring.
Similar Cre reporter strains have been produced using different promoters to express lacZ [ [ 6 7 8 9 ] ], Another variation on this theme has been the development of the Z/AP reporter strain, which switches from lacZ to alkaline phosphatase expression upon exposure to Cre recombinase activity [ 10].
A cassette coating IRES Cre SV40 pA and pgk-neomycin was cloned into this PacI site to create a targeting construct with flanking 5' and 3' genomic DNA arms of 5 kb and 2 kb, respectively.
The transfected Cre 8 cells were fed daily until there were visible plaques.
Although this approach also results in efficient targeting to a defined genomic locus it relies on co-transfection of a Cre expression plasmid along with a loxP -targeting vector that carries the transgene.