Words similar to cre

Example sentences for: cre

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More recently, transgenic mice have also been used to express Cre recombinase to mediate the conditional deletion of loxP -flanked DNA sequences in specific cell types in vivo . Traditional methods for generating transgenic mice require the availability of well characterized transcriptional regulatory elements, promoters or enhancers, that direct transgene expression in specific cell types.
β-actin/Cre mice were a gift of Dr. Gail Martin [ 23].
Briefly, recombinant adenoviruses were generated by homologous recombination in CRE8 cells, a 293 cell line that expresses Cre recombinase, between psi-5, an Ad5-derived, E1- and E3-deleted adenovirus, and pAd-lox, the adenoviral shuttle vector that expresses IL-4.
Recombination between loxP elements in ES cells was mediated by introducing a Cre expression plasmid, pHDMCCre8 (provided by Dr. Klaus Kaestner).
The recombinant virus was purified and amplified by infecting two 10 cm dishes of Cre 8 cells using 100 μl lysate.