Words similar to creb
Example sentences for: creb
How can you use “creb” in a sentence? Here are some example sentences to help you improve your vocabulary:
These findings are in accord with previous studies on mouse lung neutrophils during endotoxemia [ 21 ] . Our observation that nuclear binding activity appeared in the cells rapidly after endotoxin administration (within 0.5 hours) indicates that NF-κB and CREB are involved in early functional activation.
This was correlated with the induction of total mitogen activated protein (MAP) kinases, as well as NF-κB and cAMP response element binding (CREB) protein transcription factor activity in the cells.
CBP CREB binding protein.
We speculate that NF-κB and CREB regulate the activity of biochemical signals leading to prolonged survival and inflammatory mediator production in the lung.
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.