Example sentences for: creb

How can you use “creb” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These findings are in accord with previous studies on mouse lung neutrophils during endotoxemia [ 21 ] . Our observation that nuclear binding activity appeared in the cells rapidly after endotoxin administration (within 0.5 hours) indicates that NF-κB and CREB are involved in early functional activation.

  • This was correlated with the induction of total mitogen activated protein (MAP) kinases, as well as NF-κB and cAMP response element binding (CREB) protein transcription factor activity in the cells.

  • CBP CREB binding protein.

  • We speculate that NF-κB and CREB regulate the activity of biochemical signals leading to prolonged survival and inflammatory mediator production in the lung.

  • Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.


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