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Example sentences for: construct

How can you use “construct” in a sentence? Here are some example sentences to help you improve your vocabulary:

Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.
These alternate alignments were used to construct initial models.
Often, researchers construct a gene tree and then informally use "subtrees" (clades) to make inferences about sequences (without regard to duplications and speciations).
With high-throughput technologies and facilities, such as robotic, imaging, and computational sequencing and genotyping facilities, more reliable polymorphic markers will be used to construct a high resolution genetic map and marker-assisted selection or even map-based cloning will be realized before long in cultivated peanut.
A variation on this theme is that it might never be possible to construct a phosphosite mutant of Net1 that binds Cdc14 but fails to release it during anaphase, if the hydroxyl groups that are phosphorylated normally form critical hydrogen bonds that tether Cdc14 to Net1.