Example sentences for: constitutively

How can you use “constitutively” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To test the effect of 4EBP-1 on the proliferation of human breast cancer cells, an expression vector encoding the constitutively active 4EBP-1 mutant (4EBP-1-5A) was stably transfected into MCF7 cells.

  • In the present study an MCF7 breast cancer cell line was developed which can be induced to express a mutant form of 4EBP-1 in which five amino acids that are targets for phosphorylation have been changed to alanines [ 24 ] . Since the protein cannot be inactivated by phosphorylation it constitutively binds to and inhibits eIF4E.

  • CV-1 cells were cotransfected with a reporter plasmid responsive to androgen (ARE-TATA-luciferase) in the presence or absence of AR and constitutively active Stat3C (Fig.

  • For example, overexpression of a constitutively active form of Notch (Nac) in frog embryos led to an increase in the amount of neural tissue, even when cell division was blocked [ 29].

  • To verify equal protein loading membranes were then stripped and re-probed with the panERK antibody (1:1000; Transduction Laboratories; Lexington, KY) which recognizes the constitutively expressed ERKs in bovine CLENDO cells as previously described [ 35 ] .


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