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Example sentences for: constructed
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Alternatively, microarrays can be composed of short oligonucleotides (25 bases) synthesized directly onto a solid matrix using photolithographic technology (Affymetrix) [ 2, 9] or constructed from long oligonucleotides (55-70 bases) spotted onto glass slides [ 10, 11, 12].
That will require precise characterization of many more mutations as well as expansion of the database of mitochondrial protein profiles beyond what we have constructed using yeast as a model.
A single asparagine to aspartate substitution at position 748 in T7 RNA polymerase was already known to facilitate the utilization of the T3-like promoter [ 6 ] . A library of polymerase variants was constructed in which amino acid residues 746, 747, and 748 were completely randomized.
Phylogenetic trees [ 49 ] were constructed for each gene from amino acid data to assist in gene selection and interpretation.
We therefore constructed appropriate annular zones in the macula for percentage (multiplicative) increases in brightness to be empirically determined using published data on the spatial distribution of macular pigment [ 29 30 ] . These observers found that the macular pigment density (hence optical density) is greatest centrally, drops to half-maximum within a diameter of 500 to 600 μm, reaches a quarter-maximum at a diameter of about 1000 μm, and thence tapers slowly to a low constant level within a 2000 μm diameter region.