Words similar to conjugated
Example sentences for: conjugated
How can you use “conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Protein samples were resolved on a 10% SDS-PAGE and immunoblotted with primary antibody and revealed with horse radish peroxidase (HRP)-conjugated anti-mouse IgM, or anti-rabbit IgG (Jackson Laboratory, Bar Harbor, Maine, United States).
2-, 3- or 4-color flow cytometric analysis was performed on all samples following a standard red cell lysis method using mouse monoclonal antibodies directed against CD45 (clone 2D1, peridin-chlorophyll-a-protein [PerCP]-conjugated,) as well as various combinations of CD26 (fluorescien isothiocyanate [FITC]-conjugated), CD3 (SK7, FITC or phycoerythrin [PE]), CD4 (SK3, PE), CD7 (4H9, FITC), CD8 (SK1, FITC or PE), CD19 (SJ25C1, FITC) and CD26 (L272, FITC).
rabbit anti-p65 polyclonal (Rockland, Germany) and goat anti-rabbit horseradish peroxidase conjugated antibody (Sigma) were used as primary and secondary antibody respectively at a 1:1000 dilution.
Cy3 conjugated donkey anti-mouse or anti-rabbit secondary antibodies (1:200, Sigma) in blocking solution were applied for 1 hour.
A goat anti-mouse IgG secondary antibody conjugated to Cy3 was used at 1:200 dilution for 1 hour, regardless of which primary antibody was used.