Words similar to conjugate
Example sentences for: conjugate
How can you use “conjugate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Membranes were blocked in 5% BLOT-QuickBlocker PBST buffer (plus 0.1% Tween-20), washed three times with PBST buffer (plus 0.1% Tween-20), incubated in primary antibody diluted in femto/TBST buffer in a tray for 90 min at room temperature (or overnight at 4°C), washed five times (as above), incubated in secondary antibody-HRP (horseradish peroxidase)-conjugate (goat anti-rabbit) as above, washed four times with femto/TBST buffer followed by transfer to detection buffer and detection reagent.
Previous studies have demonstrated that these antibodies recognize the phosphorylated (active) forms of extracellular-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK) Membranes were then washed (3 × 5 min each) with TBST buffer and incubated with anti-rabbit IgG horseradish peroxidase conjugate or anti-mouse IgG horseradish peroxidase conjugate (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at room temperature.
Biotinylated cRNA was stained with 10 g/mL streptavidin-phycoerythrin conjugate (Molecular Probes) in 6X SSPE-T for 30 min at 25°C followed by 10 washes in 6xSSPE-T at 25°C.
Microplate wells coated with antigen bound human antibody, which was subsequently bound by an enzyme-labeled conjugate antibody and quantitated colorimetrically (Varelisa; Pharmacia & Upjohn, Freiburg, Germany).
The sections were washed thrice with PBS and anti-digoxigenin-peroxidase conjugate was added on the section and incubated in a humidified chamber for 30 minutes at room temperature.
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