Words similar to conjugate
Example sentences for: conjugate
How can you use “conjugate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Anti-β-actin mouse mAb (A5441), anti-β-tubulin mouse mAb (T4026), anti-vinculin mouse mAb (V9131), anti-mouse IgG FITC conjugate (F2012), anti-rabbit IgG FITC conjugate (F1262), anti-rabbit IgG TRITC conjugate (T5268), and TRITC labeled phalloidin were purchased from Sigma.
The following fluorochrome-labeled antibodies were used (AlexaFluor, Molecular Probes): goat anti-mouse IgG (H+L) conjugate (#A-11032; red), goat anti-rabbit IgG (H+L) conjugate (#A-11046; blue), and donkey anti-goat IgG (H+L) conjugate (#A-11055; green).
Subsequently, washed slides were incubated with streptavidin-peroxidase conjugate for 15 minutes, washed again, and incubated 5 minutes in substrate-chromogen solution, rinsed with PBS, washed in distilled water, counterstained 2 minutes with Harris hematoxylin diluted 1:20, dehydrated, and mounted.
Wells were washed and incubated with 100 μl of the antiserum for 2 h at 20-25°C, and incubated with 100 μl of the peroxidase conjugate for 1h.
Biotinylated mouse Ig, streptavidin/horseradish peroxidase conjugate, diamino benzidine, and Vectastain ABC were from Vector (Burlingame, CA, USA).
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