Words similar to conjugating
Example sentences for: conjugating
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COP10, which has been shown to interact with AtCOP1 in yeast two-hybrid experiment, encodes a ubiquitin conjugating enzyme (E2) variant [ 44 ] . The COP9 signalosome, an eight-subunit complex homologous to the lid subcomplex of the 26 S proteasome [ 45 ] , directly associates with SCF E3 complex and promotes deneddylation of the cullin subunit of SCF in both plant and animal cells [ 46 47 48 49 ] . The exact relationship between COP1 and the COP9 signalosome remains obscure; it is known, however, that in Arabidopsis the COP9 signalosome is required for COP1 to accumulate in the nucleus in the dark [ 29 ] . Although COP1 most likely plays a role in regulating protein degradation, attempts in reconstituting ubiquitination of HY5 by AtCOP1 have been unsuccessful.
UBC = Ubiquitin conjugating enzyme
Rabbit anti-PKA RIα was prepared by synthesizing the N-terminal 18 residues of human RIα protein and conjugating this peptide (MESGSTAASEEARSLREC) to keyhole limpet haemocyanin (KLH) via the bifunctional reagent, m-maleimidobenzoyl-N-hydroxysuccinimide ester (MES).
This system employs a cascade of enzymatic reactions that covalently attach a ubiquitin chain to substrate proteins, thereby targeting them to the proteasome [ 19 ] . The ubiquitin transfer reaction involves three enzymes: E1, which mediates the ATP-dependent activation of ubiquitin, and E2, or ubiquitin conjugating enzyme (UBC), which, together with an E3 ubiquitin ligase, transfers ubiquitin to the target protein.
In addition, we required an ubiquitin activating enzyme (E1) and an ubiquitin conjugating enzyme (UBC).
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