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Example sentences for: confluent
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Confluent cells in 96 well plates were placed in their maintenance media without serum for 24 hr and exposed to either high or low concentrations (see Results) of progesterone, β-estradiol or testosterone (all from Sigma Chemical Co., St. Louis, MO) for 1, 2 or 7 days in serum-free media.
MDCK cells transfected with empty virus showed no augmentation of transepithelial water movement, confirming previous work that showed water does not flow across tight junctions in MDCK confluent layers [ 16 ] . Cells transfected with empty virus also showed no effect of TEA treatment.
2Aand 2B, PP cultures can also be distinguished by a paucity of fibroblasts with high granularity (side scattering), a characteristic often associated with the elaboration of RER (rough endoplasmic reticulum) [ 44 ] . In both confluent and post-confluent cultures, the populations of cells with the highest granularity were found in the non-PP isolates (keloid and nonplantar).
All cell cultures were routinely passed when 90-95% confluent.
, less than 100 nM) can selectively suppress HIV-1 transcript elongation without altering cellular transcripts in cell types of various lineages, including podocytes [ 7 29 30 ] . Cyclin D 1 transcript levels were followed before, during, and after treatment of confluent wild-type or HIV-1 infected podocytes with 50 nM flavopiridol, a dose that suppresses HIV-1 transcription by 70% [ 7 ] . Analysis of HIV-1, cyclin D 1 , and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) transcript levels in podocytes treated with flavopiridol for 2 days, followed by drug washout for 1 day, showed that cyclin D 1 expression paralleled HIV-1 expression in infected podocytes before, during and after suppression of HIV-1 genes (Figure 4).
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