Words similar to confluent

Example sentences for: confluent

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Briefly, transfected CHO cells expressing the rSPR were cultured onto coverslips until ∼ 90% confluent.
Comparisons of confluent cultures with three-day postconfluent cultures revealed an increase in α-SMA levels independent of media serum content - Fig 5A(for examples of the actual FACS analyses, please, see additional file 3: Additional data #3.ppt).
Metabolic labeling of cells and immunoprecipitation was performed essentially as described previously [ 21 30 ] . In brief, confluent monolayers of A549 cells were transfected with appropriate dsRNA (at 20 nM final) as described above, and at 8 hr post-transfection, were washed with a phosphate-free buffer (120 mM NaCl, 5 mM KCl, 1.5 mM MgCl 2 , 0.25 mM CaCl 2 , 25 mM NaHCO 3 , 20 mM HEPES [pH 7.4]) and labeled with 32P-orthophosphate (500 μCi/ml) (Amersham) in Dulbecco-modified Eagle MEM lacking both phosphate and pyruvate.
Confluent cells in 96 well plates were placed in their maintenance media without serum for 24 hr and exposed to either high or low concentrations (see Results) of progesterone, β-estradiol or testosterone (all from Sigma Chemical Co., St. Louis, MO) for 1, 2 or 7 days in serum-free media.
Beta-glucuronidase endocytosis assay was carried out as described previously [ 33 ] . Briefly, confluent cell cultures were washed twice with pre-warmed serum-free DMEM followed by incubation with DMEM containing 5 mg/ml human serum albumin and 10 mM M6P for 20 minutes.