Example sentences for: confluency

How can you use “confluency” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Primary fibroblast cultures from patients and control individuals were grown to confluency and then metabolically labeled with 35S-cysteine as described [ 17 18 ] . The soluble cell lysis fraction, containing newly synthesized labeled fibrillin-1 molecules, and the insoluble fraction, representing the labeled fibrillin in the extracellular matrix, were fractionated by SDS polyacrylamide gel electrophoresis and autoradiographed.

  • Immunocytochemistry was carried out as described previously [ 40 ] . Briefly, COS cells were grown to 50% confluency on gelatin-coated glass cover slips.

  • Stably-transfected HeLa cells expressing GFP-NFAT [ 38] were grown on glass coverslips to approximately 30% confluency and either left untreated or treated with, ISIS 15534, or ISIS 15521 (a mismatched oligonucleotide control for ISIS 15534) in the absence of serum as described above.

  • For preparation of donors in G0/G1 phase, the cells were allowed to grow to confluency by culturing for 6 days and a single cell suspension was prepared by standard trypsinization.

  • Cells grown in 96-well plates (20,000 cells/well, ~90% confluency) were treated with testing agents for a period of time as indicated in the figure legends; then, the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazole-2yl)-2,5-diphenyltetrazolium bromide) assay [ 66 ] . Culture medium was removed after treatment, then 100 μl MTT solution (100 μg/ml prepared in MEM) was added to each well.


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