Example sentences for: confluency

How can you use “confluency” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Transient transfection of MDA-MB-231 breast cancer cells has been described previously [ 19 ] . Briefly, 2 μg 3TP-Luc together with 0.3 μg β-galactosidase expression plasmid were transfected into cells grown to 70-80% confluency in 6-well plates using LipofectAMINE reagent (Invitrogen) following the manufacturers protocol.

  • After 2-4 days of subculturing, the cells (approximately 50% of confluency) were cultured in the medium supplemented with 0.3 μg/ml nocodazole (Sigma), a microtubule polymerization inhibitor, for 10 h to induce G2/M phase arrest.

  • Primary fibroblast cultures from patients and control individuals were grown to confluency and then metabolically labeled with 35S-cysteine as described [ 17 18 ] . The soluble cell lysis fraction, containing newly synthesized labeled fibrillin-1 molecules, and the insoluble fraction, representing the labeled fibrillin in the extracellular matrix, were fractionated by SDS polyacrylamide gel electrophoresis and autoradiographed.

  • These cell types were all maintained in DMEM with 10% fetal calf serum with 1% antibiotic/ antimycotic, and seeded onto 24-well tissue culture plates at approximately 20,000 cells/cm 2; cultures were used immediately after reaching confluency.

  • Once 90% confluency was reached, the medium was changed and the cultures were maintained in serum- and growth factor-free conditions (EBM-2 medium) for 24 h.


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