Words similar to confluency
Example sentences for: confluency
How can you use “confluency” in a sentence? Here are some example sentences to help you improve your vocabulary:
Primary fibroblast cultures from patients and control individuals were grown to confluency and then metabolically labeled with 35S-cysteine as described [ 17 18 ] . The soluble cell lysis fraction, containing newly synthesized labeled fibrillin-1 molecules, and the insoluble fraction, representing the labeled fibrillin in the extracellular matrix, were fractionated by SDS polyacrylamide gel electrophoresis and autoradiographed.
The pCYIL-10 vector is an expression vector which was established by Dr. Xianmin Meng based on the pCY4B vector [ 40 ] . Cultured SVEC endothelial cells seeded in 24 wells were transfected at 70% confluency with SuperFect (Qiagen).
Transient transfection of MDA-MB-231 breast cancer cells has been described previously [ 19 ] . Briefly, 2 μg 3TP-Luc together with 0.3 μg β-galactosidase expression plasmid were transfected into cells grown to 70-80% confluency in 6-well plates using LipofectAMINE reagent (Invitrogen) following the manufacturers protocol.
Immunocytochemistry was carried out as described previously [ 40 ] . Briefly, COS cells were grown to 50% confluency on gelatin-coated glass cover slips.
After 2-4 days of subculturing, the cells (approximately 50% of confluency) were cultured in the medium supplemented with 0.3 μg/ml nocodazole (Sigma), a microtubule polymerization inhibitor, for 10 h to induce G2/M phase arrest.