Words similar to confluency
Example sentences for: confluency
How can you use “confluency” in a sentence? Here are some example sentences to help you improve your vocabulary:
Transient transfection of MDA-MB-231 breast cancer cells has been described previously [ 19 ] . Briefly, 2 μg 3TP-Luc together with 0.3 μg β-galactosidase expression plasmid were transfected into cells grown to 70-80% confluency in 6-well plates using LipofectAMINE reagent (Invitrogen) following the manufacturers protocol.
After 2-4 days of subculturing, the cells (approximately 50% of confluency) were cultured in the medium supplemented with 0.3 μg/ml nocodazole (Sigma), a microtubule polymerization inhibitor, for 10 h to induce G2/M phase arrest.
Primary fibroblast cultures from patients and control individuals were grown to confluency and then metabolically labeled with 35S-cysteine as described [ 17 18 ] . The soluble cell lysis fraction, containing newly synthesized labeled fibrillin-1 molecules, and the insoluble fraction, representing the labeled fibrillin in the extracellular matrix, were fractionated by SDS polyacrylamide gel electrophoresis and autoradiographed.
These cell types were all maintained in DMEM with 10% fetal calf serum with 1% antibiotic/ antimycotic, and seeded onto 24-well tissue culture plates at approximately 20,000 cells/cm 2; cultures were used immediately after reaching confluency.
Once 90% confluency was reached, the medium was changed and the cultures were maintained in serum- and growth factor-free conditions (EBM-2 medium) for 24 h.
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