Words similar to collagenase
Example sentences for: collagenase
How can you use “collagenase” in a sentence? Here are some example sentences to help you improve your vocabulary:
These include motifs for transcription factors that bind to collagenase and elastase genes and motifs for the transcription factor MEP-1, which regulates transcription from the stromelysin-1 (MMP-3) and metallothionein genes [ 18, 19].
There is disagreement over which collagenase predominates in cartilage degradation in vivo [ 10 11 ] . It is therefore of interest to find the levels of MMP-1 expressed compared with that of MMP-13 in SW-1353 and human primary chondrocytes.
Neutrophils were recovered, after digestion of the lung tissue for 25 min with 60 U/ml collagenase D (specific activity 0.22 Wunsch unit = 800 IU, low ancillary protease activity, Boehringer Mannheim, Indianapolis, IN, USA), prepared in HBSS containing 10% fetal bovine serum (FBS) and 0.01% DNAse 1 (Sigma Chemical Co., St. Louis, MO, USA), followed by filtering (220 μm).
However, immunoblot analysis using a polyclonal antibody to human MMP-1 (this reagent is not available for the chicken homologue of this matrix metalloproteinase) showed that cCAF treatment caused a decrease in the amount of the collagenase MMP-1 detected, but neither TIMP-1 nor TIMP-2 (two natural inhibitors of these MMPs) levels were changed, as evaluated by immunoblot analysis with a monoclonal antibody to the corresponding human proteins (Fig.
The epidermis was then removed with forceps and the dermal pieces were incubated with Collagenase D (3 mg/ml in collagenase buffer (see below)) for 15-30 min at 37°C.
Loading...