Words similar to co-transfection
Example sentences for: co-transfection
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Similarly, co-transfection of wild type Fyn with RlkY420F did not alter the half-life of RlkY420F in the soluble fraction (data not shown), again arguing that phosphorylation of Rlk by Fyn is required for this increased turnover in the soluble fraction.
To determine how efficiently co-transfection occurred with PEI, we used β-gal and EGFP together in a 1: 1 ratio, with the overall DNA: PEI ratio at 2 μg plasmid DNA: 5 μg PEI/ml culture media.
Stable transfected cell lines containing these constructions were selected by two methods: co-transfection of these plasmids with a second plasmid containing a hygromycin-resistance gene driven by a Herpes simplex thymidine kinase promoter or transfection of GFP-BLM allele plasmids that had been modified to contain the hygromycin-resistance gene expression unit.
Marker transfer experiments involved co-transfection of HSV-2 pol coding region (HSV-2 SB5, HSV-2 83D, HSV-2 6652 and HSV-2 6757 described in [ 21 ] ) with HP66 viral DNA into Vero cells.
The analysis of the effect of βγ was extended by examining the localization of α i2 or sα i2 after co-transfection with βγ and after treatment with ALLN.