Words similar to co-transfection
Example sentences for: co-transfection
How can you use “co-transfection” in a sentence? Here are some example sentences to help you improve your vocabulary:
The procedure for preparation of the adenovirus was co-transfection of a human embryonic kidney cell line (HEK 293) with the pACCMVpLpa plasmid and another plasmid (pJM17) containing most of the viral genome [ 30 ] . A relatively rare recombination of the two plasmids occurs in the HEK 293 cells that reconstitutes the adenoviral genome.
We show here that Ets-1 protein recognizes and binds the Ets site in the 43 bp regulatory region and that CD5 expression is positively correlated with Ets-1 levels in co-transfection studies.
While the possibility that the decreased CD5 surface expression that was demonstrated in the gene targeted mice is due to post-translational rather than transcriptional effects, our co-transfection studies with the Ets-1 expression plasmid and the CD5 promoter constructs demonstrate argue that Ets-1 is regulating transcription in all cases.
To determine how efficiently co-transfection occurred with PEI, we used β-gal and EGFP together in a 1: 1 ratio, with the overall DNA: PEI ratio at 2 μg plasmid DNA: 5 μg PEI/ml culture media.
In contrast, BCR-mediated activation of Lyn kinase was largely maintained [ 37 38 ] . The Syk-dependent signaling pathway appears to be facilitated by the adaptor molecule BLNK (also known as SLP-65 and BASH) [ 39 40 41 ] . Syk can induce the phosphorylation of BLNK in co-transfection experiments [ 42 ] , which may be important for the recruitment of other Syk substrates like PLCγ2 through a scaffolding function [ 42 ] . BLNK function is necessary for signal transduction since no Ca 2+flux or PLCγ2 phosphorylation is observed in response to BCR engagement in BLNK-deficient DT40 cells [ 43 ] .
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