Words similar to co-transfection

Example sentences for: co-transfection

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However, co-transfection of v-crk and gst-JNK with the tyrosine phosphorylated Cas chimera blocked the activation of JNK induced by v-crk (Fig.
Similarly, co-transfection of wild type Fyn with RlkY420F did not alter the half-life of RlkY420F in the soluble fraction (data not shown), again arguing that phosphorylation of Rlk by Fyn is required for this increased turnover in the soluble fraction.
Transient transfection of a Wg expression plasmid activated LEF-dependent transcription 34-fold; this result was not changed by co-transfection with an Arr plasmid (which also had no effect in the absence of Wg), implying either that Arr levels were not limiting or that Arr was not required for Wg effects.
In contrast, co-transfection with a DFz2 plasmid augmented Wg signaling more than 1000-fold above control levels and about 30-fold above levels observed with Wg alone, suggesting that DFz2 was required for efficient Wg signaling and, although detectable in S2 cells, was present in limiting amounts.
As additional controls in these experiments, we showed that inhibitory RNA against Dsh, a cytoplasmic phosphoprotein believed to mediate Wg signaling via Fz receptors, blocks about 60% of signaling produced by co-transfection with Wg and DFz2 plasmids.