Words similar to co-transformed
Example sentences for: co-transformed
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The vector was linearized between the B1 targeting hook and URA3 and co-transformed with genomic DNA into yeast spheroplasts.
These plasmids were co-transformed into yeast strain Y187 with full-length LcrV fused to the GAL4 DNA binding domain of plasmid pAS2-1 to determine if the LcrG truncations could continue to participate in an interaction with LcrV.
The genetic mobility assay was performed essentially as described in Guo et al ., 2000 (Figure 4) [ 25 ] . In brief, the donor plasmids (carrying the mutant or wild-type introns) were each co-transformed with a recipient plasmid pBRR3, carrying a promoterless tet R gene downstream of the target site (which are the ligated exons; positions -30/+15).