Words similar to co-transfected
Example sentences for: co-transfected
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cDNAs representing the GnRH receptor and RGS3 or RGS3T were co-transfected into COS-1 cells for GnRH-A treatment 72 h later; intracellular IP 3 concentrations were measured at 0, 10, 20, and 30 sec after addition of 10 -7M GnRH-A (Fig.
α s -GFP fusion proteins were assayed for the ability to stimulate adenylyl cyclase in response to luteinizing hormone (LH) receptor stimulation [ 38 ] . 10 6HEK 293 cells/60 mm-dish were co-transfected with 2 μg of plasmid DNA encoding the α s -GFP fusion protein, and 0.2 μg of plasmid DNA encoding the rat LH receptor in pCIS [ 39 ] , using 10 μL of Lipofectamine 2000.
To understand the effect of Ets-1 on CD5 expression, we co-transfected EL4 cells with increasing amounts of Ets-1 expression plasmid (pEVRF-ets-1) along with a StuI B reporter construct containing either the wild type (S) or mutated Ets binding site (E).
We co-transfected Fyn and Rlk-GFP expressing constructs into HeLa cells, and visualized Fyn with anti-Fyn serum and Texas-red conjugated secondary antibody (Fig.
To explore these issues, we used dsRNAi to reduce the levels of Arr and DFz2 RNAs in S2 cells in which Wg signaling was stimulated, since others have shown that the expression of endogenous genes in these cells can be eliminated or reduced by direct application of T7-transcribed dsRNAs [ 15 16 ] . Using this approach (Figure 2), we found that the previously observed, approximately 30-fold stimulation of luciferase activity by the Wg expression plasmid was reduced by 70% or more when S2 cells were co-transfected with inhibitory RNAs for Arr, DFz2, or both (Figure 2A), implying that endogenous levels of both types of receptor are required for an efficient response to Wg.
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