Words similar to co-transfected

Example sentences for: co-transfected

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We co-transfected Fyn and Rlk-GFP expressing constructs into HeLa cells, and visualized Fyn with anti-Fyn serum and Texas-red conjugated secondary antibody (Fig.
In experiments where β 1 and γ 2 expression plasmids were co-transfected with α i2 or sα i2 , the amounts of α, β, and γ plasmids used were 0.7, 0.2, and 0.1 μg, respectively.
To explore these issues, we used dsRNAi to reduce the levels of Arr and DFz2 RNAs in S2 cells in which Wg signaling was stimulated, since others have shown that the expression of endogenous genes in these cells can be eliminated or reduced by direct application of T7-transcribed dsRNAs [ 15 16 ] . Using this approach (Figure 2), we found that the previously observed, approximately 30-fold stimulation of luciferase activity by the Wg expression plasmid was reduced by 70% or more when S2 cells were co-transfected with inhibitory RNAs for Arr, DFz2, or both (Figure 2A), implying that endogenous levels of both types of receptor are required for an efficient response to Wg.
Thus, when co-transfected with GFP-TOP1 lacking a NLS, both full length and the N-terminal deleted BTBD2 co-localized with TOP1 to cytoplasmic bodies.
Figure 1), but both wild-type and mutant LRP6, unlike the LRP homolog Arr, activated signaling about 10- to 15-fold alone, 2- to 4-fold when co-transfected with Wg, and 4- to10-fold when co-transfected with DFz2.