Words similar to co-transfected

Example sentences for: co-transfected

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Cells were also co-transfected with EGFP (Clontech, Franklin Lakes, New Jersey, United States) to assess transfection efficiency.
Each deletion (luciferase) construct and a control LacZ reporter construct (pON405) were co-transfected into the EL4 T cell line and lysates prepared after cells were grown for 36-48 hours were assayed for luciferase activity.
Importantly, the synergistic effect of co-transfected Wg and DFz2 plasmids, approximately 1000-fold, was also dramatically reduced by addition of inhibitory RNA against Arr (Figure 2B), implying that endogenous Arr is required for the stimulatory effect of exogenous DFz2 on Wg signaling.
MDCK cells were co-transfected with EYFP-cPLA 2 and a cPLA 2 with a S505A mutation fused to ECFP (ECFP-cPLA 2 S505A).
cDNA representing the rat GnRH receptor was cloned into pcDNA3 as described and validated previously [ 22 23 ] . cDNAs representing RGS3 and RGS3T (RGS3 amino acids 314-519) were cloned into the eukaryotic expression vector, rcCMV, as described [ 24 ] . COS-1 cells cultured as detailed before [ 23 ] were co-transfected with GnRH receptor cDNA and RGS3 or RGS3T cDNA using Lipofectamine (Life Technologies, Gaithersburg, MD) [ 23 ] , and 72 h later, treated with 10 -7M GnRH-A for 0, 10, 20, or 30 sec before being collected for IP 3 measurements [ 22 23 ] .