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Example sentences for: cho
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Among genes that are stimulated by FSH/cAMP during the period of maturation of Sertoli cells (15 to 20 days of age in rats), are several subunits of cAMP-dependent protein kinase (PKA), the serine/threonine kinase mainly responsible for the downstream effects of FSH [ 15 ] . Expression of the RIIβ regulatory subunit of cAMP-dependent protein kinase is highly induced (50-fold) at the mRNA level in primary cultures of rat Sertoli cells as a late response to cAMP peaking at 12 hours [ 16 ] . We have demonstrated that expression of CAAT/Enhancer binding protein β (C/EBPβ) is induced by cAMP with rapid kinetics in Sertoli cells, and that C/EBPβ is responsible for induction of late response genes like the RIIβ-gene [ 17 ] . The RIIβ promoter contains a conserved E-box/HLH element in the basal promoter localized at -280 to -275 relative to the functional ATG [ 18 ] . This element is shown to be important for cAMP-responsiveness in granulosa cells, and it has been shown to bind both USF and Myc in CHO and NB2a cells [ 18 19 ] . In this work, we show that USF isoforms may regulate cAMP responsiveness of the RIIβ promoter by modulating the effect of C/EBP, and that the formation of USF isoforms may be regulated by cAMP in Sertoli cells.
Activation of caspase 8 was detected in CHO.asbt.
Analysis of genomic DNA isolated from treated cells showed that TCA and GCDCA induced DNA laddering in CHO.asbt.
K562 cells expressing integrin α4, α3, or α3 integrin subunit lacking a cytoplasmic tail (K562-X3C0) were described elsewhere [ 30 40 ] . Also utilized were K562 cells expressing α3 D154A, W220A and Y218A mutants [ 41 ] , and Chinese hamster ovary (CHO) cells that express human α2, α3, α5 or β1 integrin subunits [ 30 32 ] . The cDNA encoding human CD98 heavy chain [ 57 ] was provided by Jeffrey Leiden (University of Chicago) in the pUC18 vector.
The increase of LDH activity in the culture medium of CHO.asbt.
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