Words similar to cho-wt

Example sentences for: cho-wt

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Initially, we tested Chinese hamster ovary (CHO) cells that had been stably transfected with expression vectors for kinase-inactive or wild type IGF-I receptors [ 53 ] . The parental CHO cell line expresses ~50,000 endogenous IGF-IRs, the transfected wild-type (CHO-WT) cell line expresses ~250,000 IGF-IRs, and the kinase-defective (CHO-MK) cell line expresses ~250,000 mutant IGF-IRs.
Not only did we observe a difference of the number of colonies, but CHO-MK colonies were smaller than either the parental CHO or CHO-WT colonies (data not shown).
Very few apoptotic cells are seen in the CHO parental and CHO-WT cells, whereas apoptotic cells are seen throughout the tumor from the kinase-defective CHO-MK cells.
As expected, autophosphorylation of the IGF-IRs was observed in the CHO and CHO-WT cells but very little was seen in two different clones of CHO-MK cells, indicating that the kinase-defective receptors inhibited the endogenous wild-type hamster receptors (Figure 1A).