Example sentences for: centrifuged

How can you use “centrifuged” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .

  • Solubilized BBMV was centrifuged at 10,000 × g for 10 min and supernatant was concentrated to 2 ml by Amicon YM30 ultrafiltration.

  • These were made up to 20 ml in RPMI and layered again over Lymphoprep ™and centrifuged at 2000 rpm for 20 min more.

  • To prepare cell envelopes, cells were suspended in 0.01 M HEPES, pH 7.4 and sonicated on ice by using a Branson Sonifier (small tip, setting 7) [ 44 ] . The suspension was centrifuged at 12,000 x g for 2 minutes at 4°C to remove unbroken cells and debris.

  • The top layer was removed to fresh tubes for precipitation with 0.5 ml Isopropyl Alcohol, and centrifuged at 12000 rpm for 10 minutes at 4°C.


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