Words similar to centrifugation
Example sentences for: centrifugation
How can you use “centrifugation” in a sentence? Here are some example sentences to help you improve your vocabulary:
The bacteria were harvested 8 hours post-exponential phase by centrifugation (5 min, 4000 g, 4°C) and resuspended in 500 μL of cell lysis buffer (25% glucose, 10 mM EDTA, 100 mM Tris pH 7.0).
The ammonium sulfate precipitate, containing the FLAG-MHCK-C protein, was collected by centrifugation and resuspended with gentle douncing in 20 ml TBS containing 1 mM EDTA (TBSE), and 1X PIC1 and PIC2.
It is known that, when alkaline aqueous UCB is acidified to neutral or low pH's, "Usually a colloid suspension of bilirubin is formed and the solution remains clear, as observed by the naked eye, thus inviting an erroneous interpretation" [ 11 ] . Such systems, which simulate true solutions, often show sedimentation on centrifugation [ 12 13 14 15 16 ] . We here report that sedimentation of UCB from apparently clear solutions can likewise be extensive when UCB in DMSO is diluted with aqueous buffers to final mole fractions (N) of DMSO = 0.025, 0.086 and 0.31, corresponding to 9, 27, and 64 vol% of DMSO.
Subcellular fractionation of RNA into cytoskeleton-associated and cytosolic fractions was performed as previously described [ 15 34 ] . In brief, HL-60 cells were harvested by centrifugation and resuspension in buffer A (10 mM Tris pH 7.6, 0.25 M sucrose, 25 mM KCl, 5 mM MgCl 2 ) with 0.1% Igepal nonionic detergent (Sigma).
This mechanical separation of autofluorescent red blood cells allows for the imaging and counting of white blood cells from unprocessed whole blood without additional sample processing, such as centrifugation or red blood cell lysis.
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